Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum

被引:212
作者
Fan, Yijun [1 ,3 ]
He, XingJin [1 ]
Zhou, Songdong [1 ]
Luo, Aoxue [1 ,3 ]
He, Tao [2 ]
Chun, Ze [2 ]
机构
[1] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
[2] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
[3] Sichuan Agr Univ, Dujiangyan 611830, Peoples R China
关键词
Dendrobium denneanum; Polysaccharide; Antioxidant activity; Composition; Free radicals; CAPACITY; IDENTIFICATION; EXTRACTS;
D O I
10.1016/j.ijbiomac.2009.04.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three polysaccharide fractions (DDP1-1, DDP2-1 and DDP3-1) were successfully purified from the crude polysaccharide of Dendrobium denneanum by DEAE-Cellulose and Sephadex G-200 column chromatography. The average molecular weights (Mws) of these fractions were 51.5, 26.1 and 6.95 kDa, respectively. Monosaccharide components analysis indicated that DDP1-1 and DDP2-1 were composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.82:57.11:140.82:7.76 and 1.00:1.62:1.18:77.5:7.79. DDP3-1 was composed of arabinose, mannose, glucose and galactose in a molar ratio of 1.00:1.03:8.84:2.00. On the basis of antioxidant test in vitro, DDP2-1 exhibited the highest antioxidant ability among these samples. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:169 / 173
页数:5
相关论文
共 32 条
[1]   Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies [J].
Amarowicz, R ;
Pegg, RB ;
Rahimi-Moghaddam, P ;
Barl, B ;
Weil, JA .
FOOD CHEMISTRY, 2004, 84 (04) :551-562
[2]   INFRA-RED SPECTRA OF CARBOHYDRATES .1. SOME DERIVATIVES OF D-GLUCOPYRANOSE [J].
BARKER, SA ;
BOURNE, EJ ;
STACEY, M ;
WHIFFEN, DH .
JOURNAL OF THE CHEMICAL SOCIETY, 1954, (JAN) :171-176
[3]  
[陈璋辉 CHEN Zhanghui], 2005, [中国药学杂志, Chinese Pharmaceutical Journal], V40, P1781
[4]   Study on the multiple mechanisms underlying the reaction between hydroxyl radical and phenolic compounds by qualitative structure and activity relationship [J].
Cheng, ZY ;
Ren, J ;
Li, YZ ;
Chang, WB ;
Chen, ZD .
BIOORGANIC & MEDICINAL CHEMISTRY, 2002, 10 (12) :4067-4073
[5]   Fourier transform infrared spectroscopy and chemometric analysis of white wine polysaccharide extracts [J].
Coimbra, MA ;
Gonçalves, F ;
Barros, AS ;
Delgadillo, I .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (12) :3405-3411
[6]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[7]   Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp I-450 [J].
Ganesh Kumar, C ;
Joo, HS ;
Choi, JW ;
Koo, YM ;
Chang, CS .
ENZYME AND MICROBIAL TECHNOLOGY, 2004, 34 (07) :673-681
[8]  
GAO CY, 2005, LISHIZHEN MED MAT ME, V16, P725
[9]   Antioxidants from a Chinese medicinal herb -: Lithospermum erythrorhizon [J].
Han, He ;
Weng, Xinchu ;
Bi, Kaishun .
FOOD CHEMISTRY, 2008, 106 (01) :2-10
[10]   ANALYSIS OF THE MONOSACCHARIDE COMPOSITIONS OF TOTAL NON-DIALYZABLE URINARY GLYCOCONJUGATES BY THE DITHIOACETAL METHOD [J].
HONDA, S ;
SUZUKI, S ;
KAKEHI, K ;
HONDA, A ;
TAKAI, T .
JOURNAL OF CHROMATOGRAPHY, 1981, 226 (02) :341-350