Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum

被引:212
作者
Fan, Yijun [1 ,3 ]
He, XingJin [1 ]
Zhou, Songdong [1 ]
Luo, Aoxue [1 ,3 ]
He, Tao [2 ]
Chun, Ze [2 ]
机构
[1] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
[2] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
[3] Sichuan Agr Univ, Dujiangyan 611830, Peoples R China
关键词
Dendrobium denneanum; Polysaccharide; Antioxidant activity; Composition; Free radicals; CAPACITY; IDENTIFICATION; EXTRACTS;
D O I
10.1016/j.ijbiomac.2009.04.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three polysaccharide fractions (DDP1-1, DDP2-1 and DDP3-1) were successfully purified from the crude polysaccharide of Dendrobium denneanum by DEAE-Cellulose and Sephadex G-200 column chromatography. The average molecular weights (Mws) of these fractions were 51.5, 26.1 and 6.95 kDa, respectively. Monosaccharide components analysis indicated that DDP1-1 and DDP2-1 were composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.82:57.11:140.82:7.76 and 1.00:1.62:1.18:77.5:7.79. DDP3-1 was composed of arabinose, mannose, glucose and galactose in a molar ratio of 1.00:1.03:8.84:2.00. On the basis of antioxidant test in vitro, DDP2-1 exhibited the highest antioxidant ability among these samples. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:169 / 173
页数:5
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