The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans

被引:536
作者
Loftus, BJ
Fung, E
Roncaglia, P
Rowley, D
Amedeo, P
Bruno, D
Vamathevan, J
Miranda, M
Anderson, IJ
Fraser, JA
Allen, JE
Bosdet, IE
Brent, MR
Chiu, R
Doering, TL
Dontin, MJ
D'Souza, CA
Fox, DS
Grinberg, V
Fu, JM
Fukushima, M
Haas, BJ
Huang, JC
Janbon, G
Jones, SJM
Koo, HL
Krzywinski, MI
Kwon-Chung, JK
Lengeler, KB
Maiti, R
Marra, MA
Marra, RE
Mathewson, CA
Mitchell, TG
Pertea, M
Riggs, FR
Salzberg, SL
Schein, JE
Shvartsbeyn, A
Shin, H
Shumway, M
Specht, CA
Suh, BB
Tenney, A
Utterback, TR
Wickes, BL
Wortman, JR
Wye, NH
Kronstad, JW
Lodge, JK
机构
[1] Inst Genom Res, Rockville, MD 20850 USA
[2] Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA
[3] Scuola Int Super Studi Avanzati, SISSA, I-34014 Trieste, Italy
[4] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
[5] Genome Sci Ctr, Vancouver, BC V5Z 4S6, Canada
[6] Washington Univ, Lab Computat Genom, St Louis, MO 63130 USA
[7] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA
[8] St Louis Univ, Sch Med, Dept Biochem & Mol Biol, St Louis, MO 63104 USA
[9] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada
[10] Childrens Hosp, Louisiana State Hlth Sci Ctr, Dept Pediat, New Orleans, LA 70118 USA
[11] Childrens Hosp, Louisiana State Hlth Sci Ctr, Res Inst Children, New Orleans, LA 70118 USA
[12] Univ Texas, Hlth Sci Ctr, San Antonio, TX 78229 USA
[13] Inst Pasteur, Unite Mycol Mol, Paris 15, France
[14] NIAID, Mol Microbiol Sect, Clin Invest Lab, NIH, Bethesda, MD 20892 USA
[15] Univ Dusseldorf, Inst Mikrobiol, D-4000 Dusseldorf, Germany
[16] Connecticut Agr Expt Stn, New Haven, CT 06511 USA
[17] Boston Univ, Dept Med, Boston, MA 02118 USA
[18] Univ Calif Santa Cruz, Dept Biomol Engn, Santa Cruz, CA 95064 USA
[19] J Craig Venter Fdn, Joint Technol Ctr, Rockville, MD 20850 USA
关键词
D O I
10.1126/science.1103773
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cryptococcus neoformans is a basidionnycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its similar to20-megabase genome, which contains similar to6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.
引用
收藏
页码:1321 / 1324
页数:4
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