The NucleoSpin® DNA Clean-up XS kit for the concentration and purification of genomic DNA extracts: An alternative to microdialysis filtration

被引：14

作者：

Hudlow, William R. ^{[1
]}

Krieger, Robert ^{[2
]}

Meusel, Markus ^{[2
]}

Sehhat, Joshua C. ^{[1
]}

Timken, Mark D. ^{[1
]}

Buoncristiani, Martin R. ^{[1
]}

机构：

[1] Calif Dept Justice, Jan Bashinski DNA Lab, Richmond, CA 94804 USA

[2] MACHEREY NAGEL GmbH & Co KG, D-52355 Duren, Germany

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2011年 / 5卷 / 03期

关键词：

DNA; DNA extraction; PCR inhibitor; Forensic; FORENSIC CASEWORK; DEVELOPMENTAL VALIDATION; QPCR ASSAY; SAMPLES;

D O I：

10.1016/j.fsigen.2010.03.005

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Traditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon (R) and Microcon (R) centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using NucleoSpin (R) XS silica columns to concentrate and clean-up aqueous extracts from the organic extraction of DNA from biological samples. The method can be used with standard organic extraction and dithiothreitol (DTT)based differential extraction methods with no modifications to these methods prior to the concentration and clean-up step. Extracts from laboratory-prepared bloodstains, saliva and semen stains have been successfully amplified with both qPCR and STR assays. Finally, the total time to process a set of samples with the NucleoSpin (R) XS column is approximately 30 min vs. approximately 1.5 h with the Centricon (R) YM-100 filter device. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：226 / 230

页数：5

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