Atomic force microscopy as a multifunctional molecular toolbox in nanobiotechnology

被引：554

作者：

Mueller, Daniel J. ^{[1
]}

Dufrene, Yves F. ^{[2
]}

机构：

[1] Tech Univ Dresden, Ctr Biotechnol, D-01307 Dresden, Germany

[2] Catholic Univ Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium

来源：

NATURE NANOTECHNOLOGY | 2008年 / 3卷 / 05期

关键词：

D O I：

10.1038/nnano.2008.100

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

With its ability to observe, manipulate and explore the functional components of the biological cell at subnanometre resolution, atomic force microscopy (AFM) has produced a wealth of new opportunities in nanobiotechnology. Evolving from an imaging technique to a multifunctional 'lab-on-a-tip', AFM-based force spectroscopy is increasingly used to study the mechanisms of molecular recognition and protein folding, and to probe the local elasticity, chemical groups and dynamics of receptor-ligand interactions in live cells. AFM cantilever arrays allow the detection of bioanalytes with picomolar sensitivity, opening new avenues for medical diagnostics and environmental monitoring. Here we review the fascinating opportunities offered by the rapid advances in AFM.

引用

页码：261 / 269

页数：9

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