Crystallization of gankyrin, an oncoprotein that interacts with CDK4 and the S6b (rpt3) ATPase of the 19S regulator of the 26S proteasome

被引：9

作者：

Krzywda, S

Brzozowski, AM ^{[1
]}

Al-Safty, R

Welchman, R

Mee, M

Dawson, S

Fujita, J

Higashitsuji, H

Mayer, RJ

Wilkinson, AJ

机构：

[1] Univ York, Struct Biol Lab, Dept Chem, York YO10 5YW, N Yorkshire, England

[2] Univ Nottingham, Sch Med, Lab Intracellular Proteolysis, Sch Biomed Sci,Queens Med Ctr, Nottingham NG7 2UH, England

[3] Kyoto Univ, Dept Clin Mol Biol, Fac Med, Sakyo Ku, Kyoto 6068507, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903009892

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gankyrin is an oncoprotein overexpressed in hepatocarcinoma cells that binds to the cell-cycle regulator CDK4 and the S6b ATPase subunit of the regulatory component of the proteasome. It belongs to the family of ankyrin-repeat proteins that appear to mediate protein-protein interactions in diverse biochemical processes. Gankyrin has been crystallized from polyethylene glycol solutions and diffraction data have been obtained from these crystals that extend to 2.1 Angstrom spacing.

引用

收藏

页码：1294 / 1295

页数：2

相关论文

共 11 条

[1] Purification, crystallization and preliminary X-ray diffraction analysis of yeast regulatory particle non-ATPase subunit 6 (Nas6p) [J].

Adachi, N ;

Padmanabhan, B ;

Kataoka, K ;

Kijima, K ;

Yamaki, M ;

Horikoshi, M .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :859-860

[2] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[3] Clear strategy screens for macromolecular crystallization [J].

Brzozowski, AM ;

Walton, J .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2001, 34 :97-101

[4] Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome [J].

Dawson, S ;

Apcher, S ;

Mee, M ;

Higashitsuji, H ;

Baker, R ;

Uhle, S ;

Dubiel, W ;

Fujita, J ;

Mayer, RJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (13) :10893-10902

[5] Reduced stability of retinoblastoma protein by gankyrin, an oncogenic ankyrin-repeat protein overexpressed in hepatomas [J].

Higashitsuji, H ;

Itoh, K ;

Nagao, T ;

Dawson, S ;

Nonoguchi, K ;

Kido, T ;

Mayer, RJ ;

Arii, S ;

Fujita, J .

NATURE MEDICINE, 2000, 6 (01) :96-99

[6] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411

[7] Novel insights into the INK4-CDK4/6-Rb pathway: Counter action of gankyrin against INK4 proteins regulates the CDK4-mediated phosphorylation of Rb [J].

Li, JN ;

Tsai, MD .

BIOCHEMISTRY, 2002, 41 (12) :3977-3983

[8] AAA+ superfamily ATPases:: common structure-diverse function [J].

Ogura, T ;

Wilkinson, AJ .

GENES TO CELLS, 2001, 6 (07) :575-597

[9] Processing of X-ray diffraction data collected in oscillation mode [J].

Otwinowski, Z ;

Minor, W .

MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326

[10] Structure and mechanism of ATP-dependent proteases [J].

Schmidt, M ;

Lupas, AN ;

Finley, D .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (05) :584-591