Quantitative metabolomics using NMR

被引：428

作者：

Wishart, David S. ^{[1
,2
]}

机构：

[1] Univ Alberta, Dept Comp Sci, Edmonton, AB T6G 2E8, Canada

[2] Natl Inst Nanotechnol 11421, Edmonton, AB T6G 2M9, Canada

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2008年 / 27卷 / 03期

基金：

加拿大健康研究院;

关键词：

metabolic profiling; metabolism; metabolomics; metabonomics; NMR; nuclear magnetic resonance; quantitative; spectral deconvolution;

D O I：

10.1016/j.trac.2007.12.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nuclear magnetic resonance (NMR) spectroscopy can be used to both identify and quantify chemicals from complex mixtures. This can be done semi-automatically by comparing the mixture of interest to a library of reference spectra derived from pure compounds of known concentrations. This particular approach is now being exploited to characterize the metabolomes of many different biological samples in what is called quantitative metabolomics or targeted metabolic profiling. This review describes some of the practical aspects pertaining to NMR-based quantitative metabolomics and highlights some of the strengths, limitations and applications of this particular approach to metabolomics. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：228 / 237

页数：10

共 47 条

[1] The inherent accuracy of 1H NMR spectroscopy to quantify plasma lipoproteins is subclass dependent [J].

Ala-Korpela, Mika ;

Lankinen, Niko ;

Salminen, Aino ;

Suna, Teemu ;

Soininen, Pasi ;

Laatikainen, Reino ;

Ingman, Petri ;

Jauhiainen, Matti ;

Taskinen, Marja-Riitta ;

Heberger, Karoly ;

Kaski, Kimmo .

ATHEROSCLEROSIS, 2007, 190 (02) :352-358

[2] Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts [J].

Beckonert, Olaf ;

Keun, Hector C. ;

Ebbels, Timothy M. D. ;

Bundy, Jacob G. ;

Holmes, Elaine ;

Lindon, John C. ;

Nicholson, Jeremy K. .

NATURE PROTOCOLS, 2007, 2 (11) :2692-2703

[3] Curve-fitting method for direct quantitation of compounds in complex biological mixtures using 1H NMR:: Application in metabonomic toxicology studies [J].

Crockford, DJ ;

Keun, HC ;

Smith, LM ;

Holmes, E ;

Nicholson, JK .

ANALYTICAL CHEMISTRY, 2005, 77 (14) :4556-4562

[4] The comparison of plasma deproteinization methods for the detection of low-molecular-weight metabolites by 1H nuclear magnetic resonance spectroscopy [J].

Daykin, CA ;

Foxall, PJD ;

Connor, SC ;

Lindon, JC ;

Nicholson, JK .

ANALYTICAL BIOCHEMISTRY, 2002, 304 (02) :220-230

[5] Nuclear magnetic resonance lipoprotein abnormalities in prediabetic subjects in the insulin resistance atherosclerosis study [J].

Festa, A ;

Williams, K ;

Hanley, AJG ;

Otvos, JD ;

Goff, DC ;

Wagenknecht, LE ;

Haffner, SM .

CIRCULATION, 2005, 111 (25) :3465-3472

[6] Metabolomics: building on a century of biochemistry to guide human health [J].

German, J. Bruce ;

Hammock, Bruce D. ;

Watkins, Steven M. .

METABOLOMICS, 2005, 1 (01) :3-9

[7] Lipoprotein particle analysis by nuclear magnetic resonance spectroscopy [J].

Jeyarajah, Elias J. ;

Cromwell, William C. ;

Otvos, James D. .

CLINICS IN LABORATORY MEDICINE, 2006, 26 (04) :847-+

[8] Increased small low-density lipoprotein particle number - A prominent feature of the metabolic syndrome in the Framingham Heart Study [J].

Kathiresan, S ;

Otvos, JD ;

Sullivan, LM ;

Keyes, MJ ;

Schaefer, EJ ;

Wilson, PWF ;

D'Agostino, RB ;

Vasan, RS ;

Robins, SJ .

CIRCULATION, 2006, 113 (01) :20-29

[9] Metabolite fingerprinting and profiling in plants using NMR [J].

Krishnan, P ;

Kruger, NJ ;

Ratcliffe, RG .

JOURNAL OF EXPERIMENTAL BOTANY, 2005, 56 (410) :255-265

[10] Human urine as test material in 1H NMR-based metabonomics:: Recommendations for sample preparation and storage [J].

Lauridsen, Michael ;

Hansen, Steen H. ;

Jaroszewski, Jerzy W. ;

Cornett, Claus .

ANALYTICAL CHEMISTRY, 2007, 79 (03) :1181-1186

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