Thermoresponsive giant biohybrid amphiphiles

被引：57

作者：

Lavigueur, Christine ^{[2
]}

Garcia, Jordi Gonzalez ^{[2
]}

Hendriks, Linda ^{[2
]}

Hoogenboom, Richard ^{[2
]}

Cornelissen, Jeroen J. L. M. ^{[1
]}

Nolte, Roeland J. M. ^{[2
]}

机构：

[1] Univ Twente, Lab Biomol Nanotechnol, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[2] Radboud Univ Nijmegen, Inst Mol & Mat, Dept Organ Chem, NL-6500 GL Nijmegen, Netherlands

来源：

POLYMER CHEMISTRY | 2011年 / 2卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

TRANSFER RADICAL POLYMERIZATION; COFACTOR RECONSTITUTION; CYCLOADDITION REACTION; AGGREGATION BEHAVIOR; RAFT POLYMERIZATION; BLOCK-COPOLYMERS; CLICK CHEMISTRY; SMART POLYMERS; PROTEIN; MICROGELS;

D O I：

10.1039/c0py00229a

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A series of random copolymers of various lengths was prepared by atom transfer radical polymerisation (ATRP) using two acrylate monomers with short pendant ethylene glycol side chains (ethylene glycol methyl ether acrylate, EGMEA, and methoxy ethoxy ethyl acrylate, MEEA). The end group was converted to an azide to enable bioconjugation through copper-catalysed azide-alkyne cycloaddition (CuAAC). All polymers were found to be thermoresponsive, with a cloud point between 25 and 35 degrees C depending on their molecular weight. They were conjugated to enhanced green fluorescent protein (EGFP) functionalised with a single alkyne moiety, as seen by fast performance liquid chromatography (FPLC) and gel electrophoresis (SDS-PAGE). The resulting biohybrid amphiphiles were thermoresponsive. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study their self-assembly at elevated temperatures, and they were found to form spherical structures with a diameter of approximately 60 nm upon slow heating.

引用

页码：333 / 340

页数：8

共 52 条

[11] From (bio)molecules to biohybrid materials with the click chemistry approach [J].

Dirks, A. J. ;

Cornelissen, Jeroen J. L. M. ;

van Delft, Floris L. ;

van Hest, Jan C. M. ;

Nolte, Roeland J. M. ;

Rowan, Alan E. ;

Rutjes, Floris P. J. T. .

QSAR & COMBINATORIAL SCIENCE, 2007, 26 (11-12) :1200-1210

[12] Monitoring Protein-Polymer Conjugation by a Fluorogenic Cu(I)-Catalyzed Azide-Alkyne 1,3-Dipolar Cycloaddition [J].

Dirks, A. J. ;

Cornelissen, Jeroen J. L. M. ;

Nolte, Roeland J. M. .

BIOCONJUGATE CHEMISTRY, 2009, 20 (06) :1129-1138

[13] Preparation of biohybrid amphiphiles via the copper catalysed Huisgen [3+2] dipolar cycloaddition reaction [J].

Dirks, AJT ;

van Berkel, SS ;

Hatzakis, NS ;

Opsteen, JA ;

van Delft, FL ;

Cornelissen, JJLM ;

Rowan, AE ;

van Hest, JCM ;

Rutjes, FPJT ;

Nolte, RJM .

CHEMICAL COMMUNICATIONS, 2005, (33) :4172-4174

[14] Emerging applications of polymersomes in delivery: From molecular dynamics to shrinkage of tumors [J].

Discher, Dennis E. ;

Ortiz, Vanessa ;

Srinivas, Goundla ;

Klein, Michael L. ;

Kim, Younghoon ;

Christian, David ;

Cai, Shenshen ;

Photos, Peter ;

Ahmed, Fariyal .

PROGRESS IN POLYMER SCIENCE, 2007, 32 (8-9) :838-857

[15]

Hannink JM, 2001, ANGEW CHEM INT EDIT, V40, P4732, DOI 10.1002/1521-3773(20011217)40:24<4732::AID-ANIE4732>3.0.CO

[16]

2-P

[17] Synthesis of protein-polymer conjugates [J].

Heredia, Karina L. ;

Maynard, Heather D. .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (01) :45-53

[18] In situ preparation of protein - "Smart" polymer conjugates with retention of bioactivity [J].

Heredia, KL ;

Bontempo, D ;

Ly, T ;

Byers, JT ;

Halstenberg, S ;

Maynard, HD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (48) :16955-16960

[19] VOLUME-PHASE TRANSITIONS OF IONIZED N-ISOPROPYLACRYLAMIDE GELS [J].

HIROTSU, S ;

HIROKAWA, Y ;

TANAKA, T .

JOURNAL OF CHEMICAL PHYSICS, 1987, 87 (02) :1392-1395

[20] Conjugates of stimuli-responsive polymers and proteins [J].

Hoffman, Allan S. ;

Stayton, Patrick S. .

PROGRESS IN POLYMER SCIENCE, 2007, 32 (8-9) :922-932

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