A shotgun proteomic method for the identification of membrane-embedded proteins and peptides

被引：30

作者：

Blackler, Adele R. ^{[1
]}

Speers, Anna E. ^{[1
]}

Ladinsky, Mark S. ^{[2
]}

Wu, Christine C. ^{[1
]}

机构：

[1] Univ Colorado, Dept Pharmacol, Sch Med, Aurora, CO 80045 USA

[2] Univ Colorado, Boulder Lab Elect Microscopy Cells 3D, Dept MCD Biol, Boulder, CO 80309 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 07期

关键词：

proteomics; integral membrane proteins; mass spectrometry; transmembrane domains; membrane shaving; MudPIT; proteinase K; cyanogen bromide;

D O I：

10.1021/pr700795f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome.

引用

页码：3028 / 3034

页数：7

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