Murine coronavirus with an extended host range uses heparan sulfate as an entry receptor

被引:102
作者
de Haan, CAM
Li, Z
Lintelo, ET
Bosch, BJ
Haijema, BJ
Rottier, PJM
机构
[1] Univ Utrecht, Dept Infect Dis & Immunol, Div Virol, Fac Vet Med, NL-3584 CL Utrecht, Netherlands
[2] Univ Utrecht, Biomembrane Inst, NL-3584 CL Utrecht, Netherlands
关键词
D O I
10.1128/JVI.79.22.14451-14456.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Only a relatively few mutations in its spike protein allow the murine coronavirus to switch from a murine-restricted tropism to an extended host range by being passaged in vitro. One such virus that we studied had acquired two putative heparan sulfate-binding sites while preserving another site in the furin-cleavage motif. The adaptation of the virus through the use of heparan sulfate as an attachment/entry receptor was demonstrated by increased heparin binding as well as by inhibition of infection through treatment of cells and the virus with heparinase and heparin, respectively.
引用
收藏
页码:14451 / 14456
页数:6
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