Complexities in tetracycline analysis - chemistry, matrix extraction, cleanup, and liquid chromatography

被引：204

作者：

Anderson, CR ^{[1
]}

Rupp, HS ^{[1
]}

Wu, WH ^{[1
]}

机构：

[1] US FDA, Seafood Prod Res Ctr, Pacific Reg Lab NW, Bothell, WA 98021 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1075卷 / 1-2期

关键词：

tetracycline antibiotics; biological matrix extraction; chromatography;

D O I：

10.1016/j.chroma.2005.04.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The extraction and cleanup of commonly used tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) from sample matrix, and their subsequent determination via liquid chromatography can be problematic. Many manuscripts report on various challenges encountered when developing a method for tetracycline antibiotics determination. These complexities often result in less than perfect recoveries or chromatograms and are based on the underlying chemistry associated with tetracyclines. This review compiles, compares, and discusses the results and observations found in published methods, while focusing on chemical principles in order to increase the practicing chemist's understanding of TCs to aid him/her in developing useful analyses. Published by Elsevier B.V.

引用

页码：23 / 32

页数：10