A new fluorescent assay for sialyltransferase

被引：8

作者：

Kajihara, Y ^{[1
]}

Kamitani, T ^{[1
]}

Sakakibara, T ^{[1
]}

机构：

[1] Yokohama City Univ, Fac Sci, Dept Syst Funct, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan

来源：

CARBOHYDRATE RESEARCH | 2001年 / 331卷 / 04期

关键词：

sialyltransferase assay; fluorescent labeling; sialic acid;

D O I：

10.1016/S0008-6215(01)00056-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new fluorescent assay for the sialyltransferase reaction was established. After incubation of the sialyltransferase reaction, the sialyloligosaccharide obtained was treated by acid hydrolysis, and then the NeuAc that was released was labeled with 1,2-diamino-4,5-methylenedioxibenzene. The fluorescent-labeled NeuAc could be estimated by HPLC (excitation: 373 nm; emission: 448 nm) and a Lineweaver-Burk plot could be plotted with the data from this analysis. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：455 / 459

页数：5

共 17 条

[1] A CONTINUOUS SPECTROPHOTOMETRIC ASSAY FOR GLYCOSYLTRANSFERASES [J].

GOSSELIN, S ;

ALHUSSAINI, M ;

STREIFF, MB ;

TAKABAYASHI, K ;

PALCIC, MM .

ANALYTICAL BIOCHEMISTRY, 1994, 220 (01) :92-97

[2] ACTIVATION AND TRANSFER OF NOVEL SYNTHETIC 9-SUBSTITUTED SIALIC ACIDS [J].

GROSS, HJ ;

BUNSCH, A ;

PAULSON, JC ;

BROSSMER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 168 (03) :595-602

[3] ENZYMATIC INTRODUCTION OF A FLUORESCENT SIALIC-ACID INTO OLIGOSACCHARIDE CHAINS OF GLYCOPROTEINS [J].

GROSS, HJ ;

BROSSMER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (03) :583-589

[4] DETERMINATION OF MONO-O-ACETYLATED N-ACETYLNEURAMINIC ACIDS IN HUMAN AND RAT SERA BY FLUOROMETRIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

HARA, S ;

YAMAGUCHI, M ;

TAKEMORI, Y ;

FURUHATA, K ;

OGURA, H ;

NAKAMURA, M .

ANALYTICAL BIOCHEMISTRY, 1989, 179 (01) :162-166

[5] FLUOROMETRIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF N-ACETYLNEURAMINIC AND N-GLYCOLYLNEURAMINIC ACIDS AND ITS APPLICATION TO THEIR MICRODETERMINATION IN HUMAN AND ANIMAL SERA, GLYCOPROTEINS, AND GLYCOLIPIDS [J].

HARA, S ;

TAKEMORI, Y ;

YAMAGUCHI, M ;

NAKAMURA, M ;

OHKURA, Y .

ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) :138-145

[6]

HERRLER G, 1992, J BIOL CHEM, V267, P12501

[7] Enzymatic synthesis of Kdn oligosaccharides by a bacterial α-(2→6)-sialyltransferase [J].

Kajihara, Y ;

Akai, S ;

Nakagawa, T ;

Sato, R ;

Ebata, T ;

Kodama, H ;

Sato, K .

CARBOHYDRATE RESEARCH, 1999, 315 (1-2) :137-141

[8] A novel alpha-2,6-sialyltransferase: Transfer of sialic acid to fucosyl and sialyl trisaccharides [J].

Kajihara, Y ;

Yamamoto, T ;

Nagae, H ;

Nakashizuka, M ;

Sakakibara, T ;

Terada, I .

JOURNAL OF ORGANIC CHEMISTRY, 1996, 61 (24) :8632-8635

[9] A MULTIENZYME SYSTEM FOR A ONE-POT SYNTHESIS OF SIALYL T-ANTIGEN [J].

KREN, V ;

THIEM, J .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (08) :893-895

[10] A SOLID-PHASE ASSAY FOR THE ACTIVITY OF CMPNEUAC-GAL BETA-1-4GLCNAC-R ALPHA-2,6-SIALYLTRANSFERASE [J].

MATTOX, S ;

WALRATH, K ;

CEILER, D ;

SMITH, DF ;

CUMMINGS, RD .

ANALYTICAL BIOCHEMISTRY, 1992, 206 (02) :430-436

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