Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4-phosphate pathway

被引:132
作者
Guevara-García, A
San Román, C
Arroyo, A
Cortés, ME
Gutiérrez-Nava, MD
León, P
机构
[1] Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México
关键词
D O I
10.1105/tpc.104.028860
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the two building blocks for isoprenoid biosynthesis, occurs by two independent pathways in plants. The mevalonic pathway operates in the cytoplasm, and the methyl-D-erythritol 4-phosphate (MEP) pathway operates in plastids. Plastidic isoprenoids play essential roles in plant growth and development. Plants must regulate the biosynthesis of isoprenoids to fulfill metabolic requirements in specific tissues and developmental conditions. The regulatory events that modulate the plant MEP pathway are not well understood. In this article, we demonstrate that the CHLOROPLAST BIOGENESIS6 (CLB6) gene, previously shown to be required for chloroplast development, encodes 1-hydroxy-2-methyl-butenyl 4-diphosphate reductase, the last-acting enzyme of the MEP pathway. Comparative analysis of the expression levels of all MEP pathway gene transcripts and proteins in the clb6-1 mutant background revealed that posttranscriptional control modulates the levels of different proteins in this central pathway. Posttranscriptional regulation was also found during seedling development and during fosmidomycin inhibition of the pathway. Our results show that the first enzyme of the pathway, 1-deoxy-D-xylulose 5-phosphate synthase, is feedback regulated in response to the interruption of the flow of metabolites through the MEP pathway.
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收藏
页码:628 / 643
页数:16
相关论文
共 68 条
[31]   Nonsense-mediated decay of mutant waxy mRNA in rice [J].
Isshiki, M ;
Yamamoto, K ;
Satoh, H ;
Shimamoto, K .
PLANT PHYSIOLOGY, 2001, 125 (03) :1388-1395
[32]   BIOSYNTHESIS OF THE THIAZOLE MOIETY OF THIAMIN (VITAMIN-B1) IN HIGHER-PLANT CHLOROPLASTS [J].
JULLIARD, JH ;
DOUCE, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2042-2045
[33]  
JULLIARD JH, 1992, CR ACAD SCI III-VIE, V314, P285
[34]   Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of gibberellins in Arabidopsis [J].
Kasahara, H ;
Hanada, A ;
Kuzuyama, T ;
Takagi, M ;
Kamiya, Y ;
Yamaguchi, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (47) :45188-45194
[35]   Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis [J].
Kuzuyama, T ;
Takagi, M ;
Takahashi, S ;
Seto, H .
JOURNAL OF BACTERIOLOGY, 2000, 182 (04) :891-897
[36]   Direct formation of 2-C-methyl-D-erythritol 4-phosphate from 1-deoxy-D-xylulose 5-phosphate by 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a new enzyme in the non-mevalonate pathway to isopentenyl diphosphate [J].
Kuzuyama, T ;
Takahashi, S ;
Watanabe, H ;
Seto, H .
TETRAHEDRON LETTERS, 1998, 39 (25) :4509-4512
[37]   Isoprenoid biosynthesis: The evolution of two ancient and distinct pathways across genomes [J].
Lange, BM ;
Rujan, T ;
Martin, W ;
Croteau, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (24) :13172-13177
[38]   GUN4, a regulator of chlorophyll synthesis and intracellular signaling [J].
Larkin, RM ;
Alonso, JM ;
Ecker, JR ;
Chory, J .
SCIENCE, 2003, 299 (5608) :902-906
[39]   Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana [J].
Laule, O ;
Fürholz, A ;
Chang, HS ;
Zhu, T ;
Wang, X ;
Heifetz, PB ;
Gruissem, W ;
Lange, BM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6866-6871
[40]   The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants [J].
Lichtenthaler, HK .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :47-65