Fluorescence energy transfer to dye-labeled DNA from a conjugated polyelectrolyte prequenched with a water-soluble C60 derivative

被引：12

作者：

Li, Huaping ^{[1
]}

Yang, Renqiang ^{[1
]}

Bazan, Guillermo C. ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Dept Mat & Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

MACROMOLECULES | 2008年 / 41卷 / 04期

关键词：

D O I：

10.1021/ma702102v

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The emission of the cationic polyclectrolyte poly[9,9'-bis(6 ''-N,N,N-trimethylammonium)hexyl)fluorene-alt-4,7-(2,1,3-benzothiadiazole) dibromide] (PFBT) is effectively quenched via photoinduced electron transfer by the negatively charged C-60 derivative sodium diphosphorate methano[60]fullerene (SDPMF). Stern-Volmer analysis shows the quenching constant as high as 2.8 x 10(12) M-1, consistent with the formation of ground state complexes. Addition of Cy5-labeled ssDNA to PFBT leads to concurrent FRET to Cy5 and a quenching of PFBT emission that is intrinsic to the addition of ssDNA. Similar experiments carried out with a solution of PFBT that was prequenched with SDPMF result in a stable PFBT background emission and a clearly observed Cy5 emission rise. These observations were applied to ssDNA assays that take advantage of Cy5-labeled PNA. The result is a FRET-based detection method with more stable and readily quantifiable optical signals.

引用

页码：1531 / 1536

页数：6

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