A General Chemical Ligation Approach Towards Isopeptide-Linked Ubiquitin and Ubiquitin-Like Assay Reagents

被引:79
作者
Geurink, Paul P. [1 ]
El Oualid, Farid [1 ]
Jonker, Anika [1 ]
Hameed, Dharjath S. [1 ]
Ovaa, Huib [1 ]
机构
[1] Netherlands Canc Inst, Div Cell Biol, NL-1066 CX Amsterdam, Netherlands
关键词
activity assays; chemical ligation; fluorescent probes; proteases; protein-protein interactions; DEUBIQUITINATING ENZYMES; POLYUBIQUITIN BINDING; CROSS-REACTIVITY; IDENTIFICATION; PROTEINS; SPECIFICITY; CONJUGATION; UCH-L3; PROBES; CHAINS;
D O I
10.1002/cbic.201100706
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:293 / 297
页数:5
相关论文
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