Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157:H7 in raw milk and ice-cream

被引：34

作者：

Gooding, CM

Choudary, PV

机构：

[1] LAWRENCE LIVERMORE NATL LAB, CIFAR, ANTIBODY ENGN LAB, DAVIS, CA 95616 USA

[2] LAWRENCE LIVERMORE NATL LAB, DEPT ENTOMOL, DAVIS, CA 95616 USA

来源：

JOURNAL OF DAIRY RESEARCH | 1997年 / 64卷 / 01期

关键词：

D O I：

10.1017/S0022029996001896

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.

引用

页码：87 / 93

页数：7

共 17 条

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