Auxin Perception Is Required for Arbuscule Development in Arbuscular Mycorrhizal Symbiosis

被引:145
作者
Etemadi, Mohammad [1 ,2 ,3 ,4 ]
Gutjahr, Caroline [5 ]
Couzigou, Jean-Malo [1 ,2 ]
Zouine, Mohamed [3 ,4 ]
Lauressergues, Dominique [1 ,2 ]
Timmers, Antonius [6 ]
Audran, Corinne [3 ,4 ]
Bouzayen, Mondher [3 ,4 ]
Becard, Guillaume [1 ,2 ]
Combier, Jean-Philippe [1 ,2 ]
机构
[1] Univ Toulouse 3, Unite Mixte Rech 5546, Lab Rech Sci Vegetales, F-31326 Castanet Tolosan, France
[2] CNRS, Unite Mixte Rech 5546, F-31326 Castanet Tolosan, France
[3] Ecole Natl Super Agron Toulouse, Inst Natl Polytech, Genom & Biotechnol Fruits, F-31326 Castanet Tolosan, France
[4] INRA, Genom & Biotechnol Fruits, F-52627 Auzeville, France
[5] Univ Munich, Fac Biol, Genet, D-82152 Martinsried, Germany
[6] CNRS, Unite Mixte Rech 441 2594, Lab Interact Plantes Microorganismes, INRA, F-31326 Castanet Tolosan, France
关键词
LATERAL ROOT-FORMATION; MEDICAGO-TRUNCATULA ROOTS; BOX PROTEIN TIR1; PHOSPHATE TRANSPORTERS; SYSTEM ARCHITECTURE; NODULE DEVELOPMENT; PLANT HORMONES; CELL POLARITY; EMERGING ROLE; FUNGI;
D O I
10.1104/pp.114.246595
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Most land plant species live in symbiosis with arbuscular mycorrhizal fungi. These fungi differentiate essential functional structures called arbuscules in root cortical cells from which mineral nutrients are released to the plant. We investigated the role of microRNA393 (miR393), an miRNA that targets several auxin receptors, in arbuscular mycorrhizal root colonization. Expression of the precursors of the miR393 was down-regulated during mycorrhization in three different plant species: Solanum lycopersicum, Medicago truncatula, and Oryza sativa. Treatment of S. lycopersicum, M. truncatula, and O. sativa roots with concentrations of synthetic auxin analogs that did not affect root development stimulated mycorrhization, particularly arbuscule formation. DR5-GUS, a reporter for auxin response, was preferentially expressed in root cells containing arbuscules. Finally, overexpression of miR393 in root tissues resulted in down-regulation of auxin receptor genes (transport inhibitor response1 and auxin-related F box) and underdeveloped arbuscules in all three plant species. These results support the conclusion that miR393 is a negative regulator of arbuscule formation by hampering auxin perception in arbuscule-containing cells.
引用
收藏
页码:281 / U407
页数:34
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