Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum β-lactamases

被引:43
作者
Dhanji, Hiran [1 ]
Doumith, Michel [1 ]
Clermont, Olivier [2 ,3 ]
Denamur, Erick [2 ,3 ]
Hope, Russell [1 ]
Livermore, David M. [1 ]
Woodford, Neil [1 ]
机构
[1] Hlth Protect Agcy, Antibiot Resistance Monitoring & Reference Lab, Ctr Infect, London NW9 5EQ, England
[2] INSERM, U722, F-75018 Paris, France
[3] Univ Paris 07, Fac Med, F-75018 Paris, France
关键词
Escherichia coli; Epidemic; LightCycler (R) PCR; ESBL; Pandemic clone; IDENTIFICATION; ST131;
D O I
10.1016/j.ijantimicag.2010.06.007
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like. (C) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:355 / 358
页数:4
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