Removal of inhibitors of CSF-PCR to improve diagnosis of herpesviral encephalitis

被引：19

作者：

Ratnamohan, VM

Cunningham, AL

Rawlinson, WD

机构：

[1] Westmead Hosp, ICPMR, Ctr Infect Dis, Dept Virol, Westmead, NSW 2145, Australia

[2] Westmead Hosp, ICPMR, Microbiol Lab, Westmead, NSW 2145, Australia

[3] Westmead Hosp, Ctr Virus Res, Westmead Inst Hlth Res, Westmead, NSW 2145, Australia

[4] Univ Sydney, Westmead, NSW 2145, Australia

[5] Prince Wales Hosp, Dept Microbiol, Div Virol, Randwick, NSW 2031, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 72卷 / 01期

关键词：

CSF-PCR; inhibitors; herpes simplex virus; cytomegalovirus;

D O I：

10.1016/S0166-0934(98)00020-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

CSF-PCR is currently the most sensitive test to diagnose encephalitis due to cytomegalovirus or herpes simplex virus. However, false negative results sometimes arise due to inhibitors in CSF. We have shown that the inhibitory effects may be due to increased levels of proteins and increased cell numbers, but are not due to cellular DNA. Simple techniques were used to remove the inhibitors and successfully apply these methods to CSF specimens that gave equivocal results when tested untreated. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：59 / 65

页数：7

相关论文

共 17 条

[1] A POLYMERASE CHAIN-REACTION ASSAY OF CEREBROSPINAL-FLUID IN PATIENTS WITH SUSPECTED HERPES-SIMPLEX ENCEPHALITIS [J].

ANDERSON, NE ;

POWELL, KF ;

CROXSON, MC .

JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 1993, 56 (05) :520-525

[2] A PROSPECTIVE-STUDY OF THE POLYMERASE CHAIN-REACTION FOR DETECTION OF HERPES-SIMPLEX VIRUS IN CEREBROSPINAL-FLUID SUBMITTED TO THE CLINICAL VIROLOGY LABORATORY [J].

ASLANZADEH, J ;

OSMON, DR ;

WILHELM, MP ;

ESPY, MJ ;

SMITH, TF .

MOLECULAR AND CELLULAR PROBES, 1992, 6 (05) :367-373

[3] POLYMERASE CHAIN-REACTION TO DETECT CYTOMEGALOVIRUS DNA IN THE CEREBROSPINAL-FLUID OF NEONATES WITH CONGENITAL INFECTION [J].

ATKINS, JT ;

DEMMLER, GJ ;

WILLIAMSON, WD ;

MCDONALD, JM ;

ISTAS, AS ;

BUFFONE, GJ .

JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (06) :1334-1337

[4] RAPID DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS BY NESTED POLYMERASE CHAIN-REACTION ASSAY OF CEREBROSPINAL-FLUID [J].

AURELIUS, E ;

JOHANSSON, B ;

SKOLDENBERG, B ;

STALAND, A ;

FORSGREN, M .

LANCET, 1991, 337 (8735) :189-192

[5]

DECLERCQ E, 1993, J ANTIMICROB CHEMOTH, V32, P121

[6] DETECTION OF CYTOMEGALO-VIRUS IN URINE FROM NEWBORNS BY USING POLYMERASE CHAIN-REACTION DNA AMPLIFICATION [J].

DEMMLER, GJ ;

BUFFONE, GJ ;

SCHIMBOR, CM ;

MAY, RA .

JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (06) :1177-1184

[7] CSF PRETREATMENT AND THE DIAGNOSIS OF HERPES-ENCEPHALITIS USING THE POLYMERASE CHAIN-REACTION [J].

DENNETT, C ;

KLAPPER, PE ;

CLEATOR, GM ;

LEWIS, AG .

JOURNAL OF VIROLOGICAL METHODS, 1991, 34 (01) :101-104

[8] RECENT ADVANCES IN THE POLYMERASE CHAIN-REACTION [J].

ERLICH, HA ;

GELFAND, D ;

SNINSKY, JJ .

SCIENCE, 1991, 252 (5013) :1643-1651

[9] METHODS FOR ASSESSING THE STATISTICAL SIGNIFICANCE OF MOLECULAR SEQUENCE FEATURES BY USING GENERAL SCORING SCHEMES [J].

KARLIN, S ;

ALTSCHUL, SF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) :2264-2268

[10] DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS - APPLICATION OF POLYMERASE CHAIN-REACTION TO CEREBROSPINAL-FLUID FROM BRAIN-BIOPSIED PATIENTS AND CORRELATION WITH DISEASE [J].

LAKEMAN, FD ;

WHITLEY, RJ ;

ALFORD, C ;

COBBS, CG ;

MORAWETZ, R ;

SOONG, S ;

HIRSCH, MS ;

DOLAN, R ;

BETTS, R ;

REICHMAN, R ;

COREY, L ;

LEVIN, M ;

HANDLEY, D ;

CONNER, J ;

SPECTOR, S ;

OXMAN, M ;

RICHMAN, D ;

HAYDEN, F ;

GREENLEE, J ;

LINNEMANN, C ;

POWELL, D ;

DURAK, D ;

POLLARD, R ;

JONCAS, J ;

ARVIN, A ;

YEAGER, A ;

WRIGHT, P ;

EDWARDS, C ;

KLEIMAN, M ;

FIFE, K ;

LIU, K ;

HINTHORN, D ;

PAZIN, G ;

HO, M ;

BARINGER, R ;

COHEN, M ;

LEMON, S ;

CHOW, A ;

WEIL, M ;

TILLES, J .

JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (04) :857-863