Imaging of human colon cancer cells using He-Ion scanning microscopy

被引：39

作者：

Bazou, D. ^{[1
,2
]}

Behan, G. ^{[1
,2
,3
]}

Reid, C. ^{[4
]}

Boland, J. J. ^{[1
,2
,5
]}

Zhang, H. Z. ^{[1
,2
,3
]}

机构：

[1] Trinity Coll Dublin, CRANN, Dublin 2, Ireland

[2] Trinity Coll Dublin, CRANN Adv Microscopy Lab, Dublin 2, Ireland

[3] Trinity Coll Dublin, Sch Phys, Dublin 2, Ireland

[4] Trinity Coll Dublin, Ctr forMicroscopy Anal, Dublin 2, Ireland

[5] Trinity Coll Dublin, Sch Chem, Dublin 2, Ireland

来源：

JOURNAL OF MICROSCOPY | 2011年 / 242卷 / 03期

基金：

爱尔兰科学基金会;

关键词：

Helium-ion microscope; scanning electron microscope; Caco2; cells; cancer;

D O I：

10.1111/j.1365-2818.2010.03467.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Due to its low beam current and charge compensation mechanism He-Ion scanning microscopy is a very promising tool for imaging biological cells. However, to obtain relevant information, the method used for sample preparation is also critical. In this work, we have used a Carl Zeiss Orion Plus helium-ion microscope to study the effect of sample gold coating on the morphology of human colorectal adenocarcinoma Caco2 cells. The fixative glutaraldehyde was used and the selective gold coating of the samples was investigated. A comparative study with standard scanning electron microscopy is presented.

引用

页码：290 / 294

页数：5

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