Genetic characterization of accessory genes from human immunodeficiency virus type 1 group O strains

被引:22
作者
Bibollet-Ruche, F
Loussert-Ajaka, I
Simon, F
Mboup, S
Ngole, EM
Saman, E
Delaporte, E
Peeters, M [1 ]
机构
[1] ORSTOM, Retrovirus Lab, Projet SIDAK, F-34032 Montpellier 1, France
[2] CHU Bichat Claude Bernard, Virol Lab, F-75018 Paris, France
[3] Univ Cheik Anta Diop, Hop A Le Dantec, Projet SIDAK, Dakar, Senegal
[4] Ctr Hosp Mil, Yaounde, Cameroon
[5] Innogenet, B-9052 Ghent, Belgium
关键词
D O I
10.1089/aid.1998.14.951
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Human immunodeficiency virus type 1 (HIV-1) group O strains have been described as highly divergent, compared with the vast majority of the viruses involved in the worldwide AIDS pandemic, classified in group M, To gain new insights into the diversity and genetic characteristics of group O, we have sequenced the accessory gene region (from vif to vpu) of 14 isolates, Analyses of the deduced amino acid sequences for Vif, Vpr, the first exon of Tat, and Vpu indicate that most of the functional domains of these proteins, as described for group M viruses, are highly conserved and retained among all the group O strains we have characterized. The only difference concerns the Vif phosphorylation sites, which are absent in all of the group O isolates we have sequenced; in contrast, they are well conserved in nearly all of the group M isolates, in which they play critical roles in the regulation of viral replication and infectivity, As already observed for group M isolates, the Vpu protein is also highly diverse among group O strains. Phylogenetic analyses of these sequences indicate that HIV-1 group O can be separated into four different clusters, containing most of the strains we have characterized (except one, which clusters outside of the analyzed viruses). Taking into account the criteria used for clades in group M, we were not able to define group O clades definitively.
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页码:951 / 961
页数:11
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