Temperature Sensing Using Fluorescent Nanothermometers

被引：1311

作者：

Vetrone, Fiorenzo ^{[1
]}

Naccache, Rafik ^{[1
]}

Zamarron, Alicia ^{[2
]}

Juarranz de la Fuente, Angeles ^{[2
]}

Sanz-Rodriguez, Francisco ^{[2
]}

Martinez Maestro, Laura ^{[3
]}

Martin Rodriguez, Emma ^{[3
]}

Jaque, Daniel ^{[3
]}

Garcia Sole, Jose ^{[3
]}

Capobianco, John A. ^{[1
]}

机构：

[1] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H4B 1R6, Canada

[2] Univ Autonoma Madrid, Dept Biol, E-28049 Madrid, Spain

[3] Univ Autonoma Madrid, Dept Fis Mat, GIEL, E-28049 Madrid, Spain

来源：

ACS NANO | 2010年 / 4卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

nanothermometer; upconversion; HeLa cancer cell; nanoparticles; thermal sensing; UP-CONVERSION; DOPED NANOPARTICLES; THERMOMETRY; CELLS; SENSOR; NANOCRYSTALS; LUMINESCENCE; NANOSCALE; PROBES;

D O I：

10.1021/nn100244a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.

引用

页码：3254 / 3258

页数：5

共 30 条

[1] Scanning thermal imaging of microelectronic circuits with a fluorescent nanoprobe [J].