Characterization of spermatozoa surface antigens by antisperm antibodies and its influence on acrosomal exocytosis

被引：54

作者：

Bohring, C ^{[1
]}

Krause, W ^{[1
]}

机构：

[1] Univ Hosp Marburg, European Acad Androl, Clin Training Ctr, Dept Androl, Marburg, Germany

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2003年 / 50卷 / 05期

关键词：

antisperm antibodies; caspase-3; 2-D-electrophoresis; heat shock protein; infertility; isomerase ER-60;

D O I：

10.1034/j.1600-0897.2003.00103.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

PROBLEM: Antisperm antibodies (ASA) are the main cause of they impair sperm functions by binding to immunological infertility, the sperm membrane. The aim of this study was to characterize highly enriched sperm membrane proteins by 2-D-electrophoresis and to identify membrane antigens binding ASA and to evaluate the influence of ASA on the acrosome reaction (AR). METHOD OF STUDY: Sperm membrane proteins were separated by 2-D-electrophoresis and antigens were identified by immunoblotting with ASA from seminal plasma samples of infertile men. The influence of ASA on the AR were observed and determined by means of flowcytometry. RESULTS: A total of IS antigens were identified by using ASA from seminal plasma. Six of the recognized, proteins were analyzed by means of mass spectrometry and peptide matching: HSP70 and HSP70-2, disulfide-isomerase-ER60, caspase-3 and two subunits of the proteasome (component-C2 and zeta-chain). ASA from seminal plasma are able to enhance the AR in donor-spermatozoa. CONCLUSION: The biochemical identification of these proteins will be helpful to understand the mechanism by which ASA impair sperm function and the fertilization process. Spermatozoa, in which the AR was prematurely induced by ASA, will not be able to fertilize anymore.

引用

页码：411 / 419

页数：9

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