Intracellularly expressed single-domain antibody against p15 matrix protein prevents the production of porcine retroviruses

被引：35

作者：

Dekker, S

Toussaint, W

Panayotou, G

de Wit, T

Visser, P

Grosveld, F

Drabek, D

机构：

[1] Erasmus MC Rotterdam, Fac Med, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands

[2] Biomed Sci Res Ctr Alexander Fleming, Varkiza 16602, Greece

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 22期

关键词：

D O I：

10.1128/JVI.77.22.12132-12139.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence of porcine endogenous retroviruses presents a potential risk of transmission of infectious diseases (xenozoonosis) if tissues and organs from genetically modified pigs are to be used in xenotransplantation. Here, we report that intracellular expression of a llama single-domain antibody against p15, the matrix domain protein of the porcine endogenous retrovirus Gag polyprotein, blocks retrovirus production, providing the possibility of eliminating the risk of infection in xenotransplantation.

引用

收藏

页码：12132 / 12139

页数：8

相关论文

共 42 条

[11] Inhibition of Tat-mediated transactivation and HIV replication with Tat mutant and repressor domain fusion proteins [J].

Fraisier, C ;

Abraham, DA ;

van Oijen, M ;

Cunliffe, V ;

Irvine, A ;

Craig, R ;

Dzierzak, EA .

GENE THERAPY, 1998, 5 (07) :946-954

[12] HIV-1 Gag proteins: Diverse functions in the virus life cycle [J].

Freed, EO .

VIROLOGY, 1998, 251 (01) :1-15

[13] USE OF COLLOIDAL GOLD PARTICLES IN DOUBLE-LABELING IMMUNOELECTRON MICROSCOPY OF ULTRATHIN FROZEN TISSUE-SECTIONS [J].

GEUZE, HJ ;

SLOT, JW ;

VANDERLEY, PA ;

SCHEFFER, RCT .

JOURNAL OF CELL BIOLOGY, 1981, 89 (03) :653-665

[14] Monitoring xenotransplant recipients for infection by PERV [J].

Herring, C ;

Cunningham, DA ;

Whittam, AJ ;

Fernández-Suárez, XM ;

Langford, GA .

CLINICAL BIOCHEMISTRY, 2001, 34 (01) :23-27

[15] MULTISUBUNIT PROTEINS ON THE SURFACE OF FILAMENTOUS PHAGE - METHODOLOGIES FOR DISPLAYING ANTIBODY (FAB) HEAVY AND LIGHT-CHAINS [J].

HOOGENBOOM, HR ;

GRIFFITHS, AD ;

JOHNSON, KS ;

CHISWELL, DJ ;

HUDSON, P ;

WINTER, G .

NUCLEIC ACIDS RESEARCH, 1991, 19 (15) :4133-4137

[16] Determination of active single chain antibody concentrations in crude periplasmic fractions [J].

Kazemier, B ;

deHaard, H ;

Boender, P ;

vanGemen, B ;

Hoogenboom, H .

JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 194 (02) :201-209

[17] Production of α-1,3-galactosyltransferase knockout pigs by nuclear transfer coning [J].

Lai, LX ;

Kolber-Simonds, D ;

Park, KW ;

Cheong, HT ;

Greenstein, JL ;

Im, GS ;

Samuel, M ;

Bonk, A ;

Rieke, A ;

Day, BN ;

Murphy, CN ;

Carter, DB ;

Hawley, RJ ;

Prather, RS .

SCIENCE, 2002, 295 (5557) :1089-1092

[18] Two sets of human-tropic pig retrovirus [J].

LeTissier, P ;

Stoye, JP ;

Takeuchi, Y ;

Patience, C ;

Weiss, RA .

NATURE, 1997, 389 (6652) :681-682

[19] Inhibition of early and late events of the HIV-1 replication cycle by cytoplasmic fab intrabodies against the matrix protein, p17 [J].

Levin, R ;

Mhashilkar, AM ;

Dorfman, T ;

Bukovsky, A ;

Zani, G ;

Bagley, J ;

Hinkula, J ;

Niedrig, M ;

Albert, J ;

Wahren, B ;

Gottlinger, HG ;

Marasco, WA .

MOLECULAR MEDICINE, 1997, 3 (02) :96-110

[20] siRNA-directed inhibition of HIV-1 infection [J].

Novina, CD ;

Murray, MF ;

Dykxhoorn, DM ;

Beresford, PJ ;

Riess, J ;

Lee, SK ;

Collman, RG ;

Lieberman, J ;

Shankar, P ;

Sharp, PA .

NATURE MEDICINE, 2002, 8 (07) :681-686

← 1 2 3 4 5 →