A first generation bovine BAC-based physical map

被引：31

作者：

Schibler, L ^{[1
]}

Roig, A

Mahé, MF

Save, JC

Gautier, M

Taourit, S

Boichard, D

Eggen, A

Cribiu, EP

机构：

[1] INRA, Ctr Rech Jouy, Dept Genet Anim, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France

[2] INRA, Ctr Rech Jouy, Dept Genet Anim, Lab Radiobiol & Etud Genome, F-78352 Jouy En Josas, France

[3] INRA, Ctr Rech Jouy, Dept Genet Anim, Stn Genet Quantitat Appl, F-78352 Jouy En Josas, France

来源：

GENETICS SELECTION EVOLUTION | 2004年 / 36卷 / 01期

关键词：

cattle; physical map; BAC;

D O I：

10.1051/gse:2003054

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A first generation clone- based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site ( STS) marker screening. The BAC clones were selected from an Inra BAC library ( 105 984 clones) and a part of the CHORI- 240 BAC library ( 26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers ( 451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.

引用

页码：105 / 122

页数：18

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