Decrease in CD4+CD25+FoxP3+ Treg cells after pulmonary resection in the treatment of cavity multidrug-resistant tuberculosis

被引:29
作者
Wu, Ying E. [2 ]
Peng, Wen Guang [3 ]
Cai, Ying Mu [2 ]
Zheng, Gao Zhe [2 ]
Zheng, Geng Long [4 ]
Lin, Jing Hua [2 ]
Zhang, Su Wei [2 ]
Li, Ke [1 ]
机构
[1] Shantou Univ, Coll Med, Dept Prevent Med, Shantou 515031, Guangdong, Peoples R China
[2] Shantou Univ, Coll Med, Dept Lab Med, Affiliated Hosp 1, Shantou 515031, Guangdong, Peoples R China
[3] Third Peoples Hosp Shantou City, Dept Thorac Med, Shantou, Guangdong, Peoples R China
[4] Shantou Univ, Coll Med, Guangdong Key Lab Med Mol Imaging, Shantou 515031, Guangdong, Peoples R China
关键词
Multidrug-resistant tuberculosis; CD4(+)CD25(+)FoxP3(+) T-reg; Surgery; GROWTH-FACTOR-BETA; IMMUNITY;
D O I
10.1016/j.ijid.2010.04.005
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: Immune regulatory mechanisms may limit the immunopathologic condition of infection with Mycobacterium tuberculosis and suppress cellular immune responses in the host. We investigated the CD4(+)CD25(+)FoxP3(+) circulating regulatory T cells (T-reg) in patients with cavity multidrug-resistant tuberculosis (MDR-TB) before and after surgery. Methods: We compared the proportion of T-reg cells in 13 patients with cavity MDR-TB pre- and postoperatively and in 10 healthy control subjects by flow cytometry using three specific markers in peripheral blood lymphocytes: cell-surface CD4 and CD25 expression and intracellular FoxP3 expression. Results: The proportion of CD4(+)CD25(high) and CD4(+)CD25(+)FoxP3(+) T-reg was significantly higher in patients with cavity MDR-TB and at 1-month postoperatively than in healthy controls (p < 0.001). The proportion of CD4(+) and CD4(+)CD25 cells was significantly lower in patients with cavity MDR-TB than in controls (p < 0.001). Pre- and postoperative proportions of CD4(+)CD25(high) and CD4(+)CD25(+)FoxP3(+) T-reg cells showed a positive correlation (r = 0.878, p < 0.001). Conclusion: Circulating Treg cells are increased in proportion in patients with cavity MDR-TB and decreased after surgery. Infection with M. tuberculosis may induce T-reg cell-surface molecular changes with increased numbers of cells. (C) 2010 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:E815 / E822
页数:8
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