Quantitative determination and structural characterization of isoflavones in nutrition supplements by liquid chromatography-mass spectrometry

被引：33

作者：

Chen, LJ

Zhao, X ^{[1
]}

Plummer, S

Tang, J

Games, DE

机构：

[1] Sichuan Univ, Div Canc Biotherapy, State Key Lab Biotherapy, W China Med Sch,W China Hosp, Chengdu 610041, Peoples R China

[2] Sichuan Univ, Dept Obstet & Gynecol, Univ Hosp 2, W China Med Sch, Chengdu 610041, Peoples R China

[3] Univ Coll Swansea, Mass Spectrometry Res Unit, Swansea SA1 3NJ, W Glam, Wales

[4] Cultech Ltd, Swansea SA1 3NJ, W Glam, Wales

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1082卷 / 01期

关键词：

isoflavones; nutrition supplement; APCI-MS; proposed pathway fragments;

D O I：

10.1016/j.chroma.2005.03.066

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, an accurate and route method was developed to quantitative determine daidzein, genistein, glycitein, daidzin, glycitin, 6"-O-acetyldaidzin, 6"-O-acetylglycitin and 6"-O-acetylgenistin contents in selected high and low isoflavones in nutrition supplements by on line liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry (LC-APCI-MS). Improved extraction and hydrolysis methods of the isoflavones from three nutrition supplements were also studied and a rapid extraction method was developed. Comparison of different MS2 and MS3 spectra of isoflavones and some unknown compounds were also explored and proposed pathway fragments of nine isoflavones were first systematically suggested. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：60 / 70

页数：11

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