Formation and intracellular trafficking of lipoplexes and polyplexes

被引：363

作者：

Elouahabi, A ^{[1
]}

Ruysschaert, JM ^{[1
]}

机构：

[1] Free Univ Brussels, Ctr Struct Biol & Bioinformat, B-1050 Brussels, Belgium

来源：

MOLECULAR THERAPY | 2005年 / 11卷 / 03期

关键词：

D O I：

10.1016/j.ymthe.2004.12.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cationic lipid/DNA lipoplexes and cationic polymer/DNA polyplexes represent an attractive alternative to viral vectors for cell transfection in vitro and in vivo but still suffer from a relatively low efficiency. Optimization of their transfection efficiency may be attempted by using a trial and error approach consisting of synthesizing and testing a large number of derivatives. On the other hand, rational design of highly efficient cationic lipids and polymers requires a deeper understanding of the interactions between the vector and the DNA as well as the cellular pathways and mechanisms involved in DNA entry into the cell and ultimately the nucleus. in the present review, the pathways and mechanisms involved in lipoplex- and polyplex-mediated transfection are comparatively addressed and unresolved questions are highlighted.

引用

页码：336 / 347

页数：12

共 99 条

[31] LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].

FELGNER, PL ;

GADEK, TR ;

HOLM, M ;

ROMAN, R ;

CHAN, HW ;

WENZ, M ;

NORTHROP, JP ;

RINGOLD, GM ;

DANIELSEN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7413-7417

[32] Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes [J].

Friend, DS ;

Papahadjopoulos, D ;

Debs, RJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1996, 1278 (01) :41-50

[33] MODE OF FORMATION AND STRUCTURAL FEATURES OF DNA CATIONIC LIPOSOME COMPLEXES USED FOR TRANSFECTION [J].

GERSHON, H ;

GHIRLANDO, R ;

GUTTMAN, SB ;

MINSKY, A .

BIOCHEMISTRY, 1993, 32 (28) :7143-7151

[34] COMPLEXES BETWEEN CATIONIC LIPOSOMES AND DNA VISUALIZED BY CRYO-TEM [J].

GUSTAFSSON, J ;

ARVIDSON, G ;

KARLSSON, G ;

ALMGREN, M .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1235 (02) :305-312

[35] Liposomes composed of a double-chain cationic amphiphile (Vectamidine) induce their own encapsulation into human erythrocytes [J].

Hägerstrand, H ;

Danieluk, M ;

Bobrowska-Hägerstrand, M ;

Pector, V ;

Ruysschaert, JM ;

Kralj-Iglic, V ;

Iglic, A .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1421 (01) :125-130

[36]

Hagstrom JE, 1997, J CELL SCI, V110, P2323

[37] Lipid-DNA complex formation: Reorganization and rupture of lipid vesicles in the presence of DNA as observed by cryoelectron microscopy [J].

Huebner, S ;

Battersby, BJ ;

Grimm, R ;

Cevc, G .

BIOPHYSICAL JOURNAL, 1999, 76 (06) :3158-3166

[38] Nuclear-associated plasmid, but not cell-associated plasmid, is correlated with transgene expression in cultured mammalian cells [J].

James, MB ;

Giorgio, TD .

MOLECULAR THERAPY, 2000, 1 (04) :339-346

[39] Visualization of intracellular trafficking of exogenous DNA delivered by cationic liposomes [J].

Kamiya, H ;

Fujimura, Y ;

Matsuoka, I ;

Harashima, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 298 (04) :591-597

[40] Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative [J].

Kawaura, C ;

Noguchi, A ;

Furuno, T ;

Nakanishi, M .

FEBS LETTERS, 1998, 421 (01) :69-72

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