A yeast model for the study of human DFNA5, a gene mutated in nonsyndromic hearing impairment

被引：37

作者：

Gregan, J

Van Laer, L

Lieto, LD

Van Camp, G

Kearsey, SE

机构：

[1] Univ Oxford, Dept Zool, Oxford OX1 3PS, England

[2] Univ Antwerp, Dept Med Genet, B-2020 Antwerp, Belgium

[3] Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2003年 / 1638卷 / 02期

关键词：

cell cycle; MCM10; Schizosaccharomyces pombe; hearing impairment; DFNA5; yeast;

D O I：

10.1016/S0925-4439(03)00083-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mutation in human DFNA5 is associated with autosomal dominant nonsyndromic hearing impairment. The function of DFNA5 protein remains unknown and no experimental model has been described so far. Here we describe fission yeast Schizosaccharomyces pombe as a model organism for studying the function of heterologously expressed DFNA5. We have expressed wild-type as well as mutant DFNA5 alleles under control of regulatable nmt1 promoter. Yeast cells tolerated expression of wild-type DFNA5, while expression of the mutant DFNA5 allele, which is responsible for nonsyndromic autosomal dominant hearing impairment, led to cell cycle arrest. We identified new rat and horse DFNA5 homologues and we describe a domain of homology shared between DFNA5 and the Mcm 10 family of DNA replication proteins. Genetic interactions between heterologously expressed DFNA5 and a fission yeast cdc23 (mcm10) mutant support a possible link between DFNA5 and Mcm10 proteins. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：179 / 186

页数：8

共 23 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, SF
Madden, TL
Schaffer, AA
Zhang, JH
Zhang, Z
Miller, W
Lipman, DJ
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
[2] The essential Schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene
Aves, SJ
Tongue, N
Foster, AJ
Hart, EA
[J]. CURRENT GENETICS, 1998, 34 (03) : 164 - 171
[3] Dissection of a nuclear localization signal
Hodel, MR
Corbett, AH
Hodel, AE
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (02) : 1317 - 1325
[4] The PROSITE database, its status in 1999
Hofmann, K
Bucher, P
Falquet, L
Bairoch, A
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (01) : 215 - 219
[5] Homesley L, 2000, GENE DEV, V14, P913
[6] Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae
Kawasaki, Y
Hiraga, S
Sugino, A
[J]. GENES TO CELLS, 2000, 5 (12) : 975 - 989
[7] Modulation of DNA topoisomerase II activity and expression in melanoma cells with acquired drug resistance
Lage, H
Helmbach, H
Dietel, M
Schadendorf, D
[J]. BRITISH JOURNAL OF CANCER, 2000, 82 (02) : 488 - 491
[8] Lage H, 1999, INT J CANCER, V80, P744, DOI 10.1002/(SICI)1097-0215(19990301)80:5<744::AID-IJC19>3.0.CO
[9] 2-5
[10] DFNA5 (ICERE-1) contributes to acquired etoposide resistance in melanoma cells
Lage, H
Helmbach, H
Grottke, C
Dietel, M
Schadendorf, D
[J]. FEBS LETTERS, 2001, 494 (1-2) : 54 - 59

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