Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells

被引：449

作者：

Kim, Kitai ^{[1
,2
,3
,4
]}

Zhao, Rui ^{[1
,2
,3
,4
]}

Doi, Akiko ^{[5
,6
]}

Ng, Kitwa ^{[1
,2
,4
]}

Unternaehrer, Juli ^{[1
,2
,3
,4
]}

Cahan, Patrick ^{[1
,2
,3
,4
]}

Huo Hongguang ^{[1
,2
,4
]}

Loh, Yuin-Han ^{[1
,2
,3
,4
]}

Aryee, Martin J. ^{[7
]}

Lensch, M. William ^{[1
,2
,3
,4
]}

Li, Hu ^{[8
,9
]}

Collins, James J. ^{[8
,9
,10
]}

Feinberg, Andrew P. ^{[5
,6
]}

Daley, George Q. ^{[1
,2
,3
,4
,11
]}

机构：

[1] Childrens Hosp, Howard Hughes Med Inst, Stem Cell Transplantat Program, Div Pediat Hematol Oncol,Manton Ctr Orphan Dis Re, Boston, MA 02115 USA

[2] Dana Farber Canc Inst, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[4] Harvard Stem Cell Inst, Cambridge, MA USA

[5] Johns Hopkins Univ, Sch Med, Ctr Epigenet, Baltimore, MD USA

[6] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA

[7] Johns Hopkins Univ, Dept Oncol, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA

[8] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[9] Boston Univ, Ctr BioDynam, Boston, MA 02215 USA

[10] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[11] Brigham & Womens Hosp, Div Hematol, Boston, MA 02115 USA

来源：

NATURE BIOTECHNOLOGY | 2011年 / 29卷 / 12期

基金：

美国国家卫生研究院;

关键词：

METHYLATION; MEMORY;

D O I：

10.1038/nbt.2052

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.

引用

页码：1117 / 1119

页数：3

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