A microbial chip combined with scanning electrochemical microscopy

被引：22

作者：

Kaya, T

Nishizawa, M

Yasukawa, T

Nishiguchi, M

Onouchi, T

Matsue, T ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan

[2] Matsushita Seiko Co Ltd, Kasugai, Aichi 4868522, Japan

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 04期

关键词：

bactericide; microbial chip; respiration activity; scanning electrochemical microscopy (SECM);

D O I：

10.1002/bit.10085

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was prepared by spotting a suspension of Escherichia coli on a polystyrene substrate by using a glass capillary pen. The respiration activity of the E. coli spot was imaged with SECM by mapping the oxygen concentration around the spot. The SECM images of the microbial chips clearly showed spots with lower reduction currents, indicating that E. coli in the spots uptake oxygen by respiration. The bactericidal effects of antibiotics (streptomycin and ampicillin) were measured using the E. coli-based microbial chip, and discussed in comparison with the minimum inhibitory concentration (MIC) determined by an agar plate dilution method. (C) 2001 John Wiley & Sons, Inc.

引用

页码：391 / 394

页数：4

共 24 条

[1] EFFECT OF STREPTOMYCIN ON SYNTHESIS OF PROLINE IN ESCHERICHIA COLI [J].