Adjuvant and immunostimulating properties of the recombinant Bm86 protein expressed in Pichia pastoris

The cattle tick Boophilus microplus has remained a Intent problem to the cattle industry. The recombinant vaccine GAVAC(TM) against the cattle tick has proved its efficacy and, conveniently, combined with the use of chemicals could be the solution to this problem. As this vaccine is based in the recombinant concealed antigen Bm86, if has to be given periodically to the animal to maintain an adequate level of antibodies. Some other commercially available vaccines for-cattle also have to be given periodically which creates the possibility of combining vaccines for cattle. In an attempt to evaluate the possible interactions of the Bm86 with other vaccine antigens, a potent stimulatory effect was demonstrated of the recombinant Bm86 on the humoral immune response to the recombinant Hepatitis B surface antigen in mice, and to the inactivated Infectious Bovine Rhinothraqueitis virus in cattle. These results make the Bm86 antigen expressed in Pichia pastoris a good candidate for combining vaccines for cattle because of its dual role, immunogen and adjuvant. (C) 1998 Elsevier Science Ltd. All rights reserved.