Bacterial repression loops require enhanced DNA flexibility

被引:101
作者
Becker, NA
Kahn, JD
Maher, LJ
机构
[1] Mayo Clin, Coll Med, Dept Biochem & Mol Biol, Rochester, MN 55905 USA
[2] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
关键词
lac operon; lac repressor; DNA looping; HU; HMG;
D O I
10.1016/j.jmb.2005.04.035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli lac operon provides a classic paradigm for understanding regulation of gone transcription. It is now appreciated that lac promoter repression involves cooperative binding of the bidentate lac repressor tetramer to pairs of lac operators via DNA looping. We have adapted components of this system to create in artificial assay of DNA flexibility in E. coli. This approach allows for systematic study of endogenous and exogenous proteins as architectural factors that enhance apparent DNA flexibility in vivo. We show that inducer binding does not completely remove repression loops but it does alter their geometries. Deletion of the E. coli HU protein drastically destabilizes small repression loops, an effect that can be partially overcome by expression of a heterologous mammalian HMG protein. These results emphasize that the inherent torsional inflexibility of DNA restrains looping and must be modulated in vivo. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:716 / 730
页数:15
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