The dependence of algal H2 production on Photosystem II and O2 consumption activities in sulfur-deprived Chlamydomonas reinhardtii cells

被引:132
作者
Antal, TK
Krendeleva, TE
Laurinavichene, TV
Makarova, VV
Ghirardi, ML
Rubin, AB
Tsygankov, AA
Seibert, M
机构
[1] Basic Sci Ctr, Natl Renewable Energy Lab, Golden, CO 80401 USA
[2] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119899, Russia
[3] RAS, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2003年 / 1607卷 / 2-3期
基金
俄罗斯基础研究基金会;
关键词
Chlamydomonas reinhardtii; photosystem II; O-2; consumption;
D O I
10.1016/j.bbabio.2003.09.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chlamydomonas reinhardtii cultures, deprived of inorganic sulfur, undergo dramatic changes during adaptation to the nutrient stress [Biotechnol. Bioeng. 78 (2002) 73 1]. When the capacity for Photosystem II (PSII) O-2 evolution decreases below that of respiration, the culture becomes anaerobic [Plant Physiol. 122 (2000) 127]. We demonstrate that (a) the photochemical activity of PSII, monitored by in situ fluorescence, also decreases slowly during the aerobic period; (b) at the exact time of anaerobiosis, the remaining PSII activity is rapidly down regulated; and (c) electron transfer from PSII to PSI abruptly decreases at that point. Shortly thereafter, the PSII photochemical activity is partially restored, and H-2 production starts. Hydrogen production, which lasts for 3-4 days, is catalyzed by an anaerobically induced, reversible hydrogenase. While most of the reductants used directly for H-2 gas photoproduction come from water, the remaining electrons must come from endogenous substrate degradation through the NAD(P)H plastoquinone (PQ) oxido-reductase pathway. We propose that the induced hydrogenase activity provides a sink for electrons in the absence of other alternative pathways, and its operation allows the partial oxidation of intermediate photosynthetic carriers, including the PQ pool, between PSII and PSI. We conclude that the reduced state of this pool, which controls PSII photochemical activity, is one of the main factors regulating H-2 production under sulfur-deprived conditions. Residual O-2 evolved under these conditions is probably consumed mostly by the aerobic oxidation of storage products linked to mitochondrial respiratory processes involving both the cytochrome oxidase and the alternative oxidase. These functions maintain the intracellular anaerobic conditions required to keep the hydrogenase enzyme in the active, induced form. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:153 / 160
页数:8
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