Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes

被引:196
作者
Myler, PJ
Audleman, L
DeVos, T
Hixson, G
Kiser, P
Lemley, C
Magness, C
Rickel, E
Sisk, E
Sunkin, S
Swartzell, S
Westlake, T
Bastien, P
Fu, GL
Ivens, A
Stuart, K
机构
[1] Seattle Biomed Res Inst, Seattle, WA 98109 USA
[2] Univ Washington, Dept Pathobiol, Seattle, WA 98195 USA
[3] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
[4] Fac Med, Parasitol Lab, F-34090 Montpellier, France
[5] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
[6] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, England
基金
英国惠康基金;
关键词
D O I
10.1073/pnas.96.6.2902
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Leishmania are evolutionarily ancient protozoans (Kinetoplastidae) and important human pathogens that cause a spectrum of diseases ranging from the asymptomatic to the lethal. The Leishmania genome is relatively small [approximate to 34 megabases (Mb)], lacks substantial repetitive DNA, and is distributed among 36 chromosomes pairs ranging in size from 0.3 Mb to 2.5 Mb, making it a useful candidate for complete genome sequence determination. We report here the nucleotide sequence of the smallest chromosome, chr1. The sequence of chr1 has a 257-kilobase region that is densely packed with 79 protein-coding genes. This region is flanked by telomeric and subtelomeric repetitive elements that vary in number and content among the chr1 homologs, resulting in an approximate to 27.5-kilobase size difference. Strikingly, the first 29 genes are all encoded on one DNA strand, whereas the remaining 50 genes are encoded on the opposite strand. Based on the gene density of chr1, ne predict a total of approximate to 9,800 genes in Leishmania, of which 40% may encode unknown proteins.
引用
收藏
页码:2902 / 2906
页数:5
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