A quantum-dot based protein module for in vivo monitoring of protease activity through fluorescence resonance energy transfer

被引：36

作者：

Biswas, Payal ^{[2
]}

Cella, Lakshmi N. ^{[1
,3
]}

Kang, Seung Hyun ^{[2
]}

Mulchandani, Ashok ^{[2
]}

Yates, Marylynn V. ^{[4
]}

Chen, Wilfred ^{[1
]}

机构：

[1] Univ Delaware, Dept Chem Engn, Newark, DE 19716 USA

[2] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

[3] Univ Calif Riverside, Cell Mol & Dev Biol Grad Program, Riverside, CA 92521 USA

[4] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 18期

基金：

美国国家科学基金会;

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; MATRIX METALLOPROTEINASES; ELASTIN-PROTEIN; HIV-1; PROTEASE; INHIBITORS; RESISTANCE; TARGETS; PHASE; VPR;

D O I：

10.1039/c1cc10648a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we present a new generation of nanoscale probes for in vivo monitoring of protease activity by fluorescence resonance energy transfer (FRET). The approach is based on a genetically programmable protein module carrying a fluorescently labeled, protease-specific sequence that can self-assemble onto quantum dots. The protein module was used for real-time detection of human immunodeficiency virus type-1 protease (HIV-1 Pr) activity as well as quantitative assessment of inhibitor efficiency.

引用

页码：5259 / 5261

页数：3

共 29 条

[1]

Abbenante Giovanni, 2005, Med Chem, V1, P71, DOI 10.2174/1573406053402569

[2] Dynamic imaging of protease activity with fluorescently quenched activity-based probes [J].

Blum, G ;

Mullins, SR ;

Keren, K ;

Fonovic, M ;

Jedeszko, C ;

Rice, MJ ;

Sloane, BF ;

Bogyo, M .

NATURE CHEMICAL BIOLOGY, 2005, 1 (04) :203-209

[3] Resistance to human immunodeficiency virus type 1 protease inhibitors [J].

Boden, D ;

Markowitz, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (11) :2775-2783

[4]

BRYD CM, 2006, DRUG DEVELOP RES, V67, P501

[5] Model system for high-throughput screening of novel human immunodeficiency virus protease inhibitors in Escherichia coli [J].

Cheng, TJ ;

Brik, A ;

Wong, CH ;

Kan, CC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (07) :2437-2447

[6] Suppression of HIV-1 protease inhibitor resistance by phosphonate-mediated solvent anchoring [J].

Cihlar, Tomas ;

He, Gong-Xin ;

Liu, Xiaohong ;

Chen, James M. ;

Hatada, Marcos ;

Swaminathan, Swami ;

McDermott, Martin J. ;

Yang, Zheng-Yu ;

Mulato, Andrew S. ;

Chen, Xiaowu ;

Leavitt, Stephanie A. ;

Stray, Kirsten M. ;

Lee, William A. .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 363 (03) :635-647

[7] Controlling apoptosis by inhibition of caspases [J].

Concha, NO ;

Abdel-Meguid, SS .

CURRENT MEDICINAL CHEMISTRY, 2002, 9 (06) :713-726

[8] VPR IS REQUIRED FOR EFFICIENT REPLICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN MONONUCLEAR PHAGOCYTES [J].

CONNOR, RI ;

CHEN, BK ;

CHOE, S ;

LANDAU, NR .

VIROLOGY, 1995, 206 (02) :935-944

[9] Quantum dots: a powerful tool for understanding the intricacies of nanoparticle-mediated drug delivery [J].

Delehanty, James B. ;

Boeneman, Kelly ;

Bradburne, Christopher E. ;

Robertson, Kelly ;

Medintz, Igor L. .

EXPERT OPINION ON DRUG DELIVERY, 2009, 6 (10) :1091-1112

[10] Intracellular delivery of large molecules and small particles by cell-penetrating proteins and peptides [J].

Gupta, B ;

Levchenko, TS ;

Torchilin, VP .

ADVANCED DRUG DELIVERY REVIEWS, 2005, 57 (04) :637-651

← 1 2 3 →