Isolation and characterization of ethanol-producing yeasts from fruits and tree barks

被引：59

作者：

Rao, R. S. ^{[1
]}

Bhadra, B. ^{[1
]}

Shivaji, S. ^{[1
]}

机构：

[1] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2008年 / 47卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.2008.02380.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: Isolation and identification of yeasts converting xylose to ethanol. Methods and Results: A total of 374 yeasts were isolated from a variety of rotten fruits and barks of trees. Out of these, 27 yeast strains were able to assimilate xylose and produce 0.12-0.38 g of ethanol per gram of xylose. Based on phylogenetic analysis of D1/D2 domain sequence of LSU (Large Subunit) rRNA gene and phenotypic characteristics the ethanol-producing strains were identified as member(s) of the genera Pichia, Candida, Kluyveromyces, Issatchenkia, Zygosacchraomyces, Clavispora, Debaryomyces, Metschnikowia, Rhodotorula and Cryptococcus. Conclusion: Yeast strains producing ethanol from xylose have been isolated from a variety of rotten fruits and barks of trees and identified. Significance and Impact of the Study: Environmental isolates of yeasts which could convert xylose to ethanol could form the basis for bio-fuel production and proper utilization of xylan rich agricultural and forest wastes.

引用

页码：19 / 24

页数：6

共 31 条

[21] Xylitol production by Candida sp.:: parameter optimization using Taguchi approach [J].

Rao, RS ;

Prakasham, RS ;

Prasad, KK ;

Rajesham, S ;

Sarma, PN ;

Rao, LV .

PROCESS BIOCHEMISTRY, 2004, 39 (08) :951-956

[22]

RAO RS, 2007, P AP ACAD SCI, V10, P91

[23] CONVERSION OF D-XYLOSE INTO ETHANOL BY THE YEAST PACHYSOLEN-TANNOPHILUS [J].

SCHNEIDER, H ;

WANG, PY ;

CHAN, YK ;

MALESZKA, R .

BIOTECHNOLOGY LETTERS, 1981, 3 (02) :89-92

[24]

Silva E. G., 2005, FEMS YEAST RES, V5, P859

[25] CONVERSION OF D-XYLOSE TO ETHANOL BY THE YEAST PACHYSOLEN-TANNOPHILUS [J].

SLININGER, PJ ;

BOTHAST, RJ ;

VANCAUWENBERGE, JE ;

KURTZMAN, CP .

BIOTECHNOLOGY AND BIOENGINEERING, 1982, 24 (02) :371-384

[26] Production of ethanol from wood hydrolyzate by yeasts [J].

Sreenath, HK ;

Jeffries, TW .

BIORESOURCE TECHNOLOGY, 2000, 72 (03) :253-260

[27] Wood ingestion by passalid beetles in the presence of xylose-fermenting gut yeasts [J].

Suh, SO ;

Marshall, CJ ;

McHugh, JV ;

Blackwell, M .

MOLECULAR ECOLOGY, 2003, 12 (11) :3137-3145

[28] Continuous cultivation of dilute-acid hydrolysates to ethanol by immobilized Saccharomyces cerevisiae [J].

Taherzadeh, MJ ;

Millati, R ;

Niklasson, C .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2001, 95 (01) :45-57

[29] The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].

Thompson, JD ;

Gibson, TJ ;

Plewniak, F ;

Jeanmougin, F ;

Higgins, DG .

NUCLEIC ACIDS RESEARCH, 1997, 25 (24) :4876-4882

[30] ALCOHOLIC FERMENTATION OF DEUTERIUM-XYLOSE BY YEASTS [J].

TOIVOLA, A ;

YARROW, D ;

VANDENBOSCH, E ;

VANDIJKEN, JP ;

SCHEFFERS, WA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (06) :1221-1223

← 1 2 3 4 →