A short, novel, and cheaper procedure for oligonucleotide synthesis using automated solid phase synthesizer

被引：24

作者：

Song, QL ^{[1
]}

Wang, ZW ^{[1
]}

Sanghvi, YS ^{[1
]}

机构：

[1] ISIS Pharmaceut Inc, Carlsbad, CA 92008 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

antisense; sulfur-transfer reagent; dimethylthiuram disulfide; oligonucleotide synthesis;

D O I：

10.1081/NCN-120021968

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dimethylthiuram disulfide (DTD) has been developed as an efficient thiolation reagent during automated synthesis of oligonucleotides using phosphoramidite chemistry. Simultaneous thiolation and capping was accomplished by mixing DTD with capping solution B, which saved 20% of solvent consumption and compressed the four-step synthesis cycle to three. Large-scale (1 mmol) synthesis of phosphorothioate oligonucleotides has been demonstrated with excellent yield and purity.

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收藏

页码：629 / 633

页数：5

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