Sulphur-containing amino acids are agonists for group 1 metabotropic receptors expressed in clonal RCT cell lines

Comparison of the pharmacological effects of a range of sulphur-containing amino acids on human mGluR1 alpha and mGluR5a has been undertaken, cDNAs of each mGluR were transfected into a Syrian hamster tumour cell line AV12-664 that was previously transfected with the rat glutamate-aspartate transporter protein (GLAST). The L-isomers of cysteine sulphinic acid (CSA), homocysteine sulphinic acid (HCSA), cysteic acid (CA) and serine-O-sulphate (SOS) stimulated PI hydrolysis in human mGluR1 alpha and mGluR5a cells with full agonist effects. D-CSA, the only active D-isomer, was a partial agonist for mGluR5a whereas L-sulphocysteine (S-CYS) showed weak agonist-like effects at high concentrations on both mGluR1 alpha and mGluR5a. L-Homocysteic acid was inactive on both mGluR1 alpha and mGluR5a cells. Treatment of mGluR cultures with glutamate pyruvate transaminase did not alter the potencies of the S-amino acids on PI hydrolysis responses. Inhibitor constants (K-i) obtained for L-HCSA, L-CSA, L-CA and L-SOS in [H-3]glutamate receptor binding studies with mGluR1 alpha cells indicated that L-HCSA, L-CSA, L-CA and L-SOS can bind specifically to mGluR1 with L-HCSA showing the highest affinity. These results confirm that certain endogenously produced S-amino acids may interact directly with group 1 mGluRs. (C) 1998 Elsevier Science Ltd. All rights reserved.