Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes

被引：18

作者：

Su, Hongyan ^{[1
]}

Wang, Lei ^{[1
]}

Liu, Linde ^{[1
]}

Chi, Xiaoyan ^{[1
]}

Zhang, Yuxiang ^{[1
]}

机构：

[1] Ludong Univ, Coll Life Sci, Yantai 264025, Peoples R China

来源：

JOURNAL OF APPLIED GENETICS | 2008年 / 49卷 / 03期

关键词：

edible mushroom; Flammulina velutipes; SCAR markers; strain specificity;

D O I：

10.1007/BF03195619

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper, we report for the first time oil authentication of Flammulina velutipes cultivars by using strain-specific sequence-characterized amplified region (SCAR) markers developed from inter-simple sequence repeat (ISSR) markers. The genomic DNA polymorphism was analyzed by the ISSR technique in 7 strains of F. velutipes presently cultivated in China on a commercial scale. Eight primers selected from 20 ISSR primers amplified 104 clear and stable bands, of which 81 bands were polymorphic. Among the selected primers, primer ISSR9 can distinguish strain No. 12 from the other 6 strains by amplifying a unique and reproducible band of approximately 750 bp. According to the sequence of the strain-specific fragment, a pair of SCAR primers was designed to diagnose strain No. 12 on the molecular level. The validity of the SCAR marker was confirmed by using DNA samples from another 12 strains of F. velutipes obtained from different parts of China. Our data provided the foundation for a precise and rapid PCR-based strain-diagnostic system for F. Velutipes.

引用

页码：233 / 235

页数：3

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