The carboxyl terminus of protein TraD adds specificity and efficiency to F-plasmid conjugative transfer

被引:54
作者
Sastre, JI [1 ]
Cabezón, E [1 ]
de la Cruz, F [1 ]
机构
[1] Univ Cantabria, Dept Mol Biol, Santander 39011, Spain
关键词
D O I
10.1128/JB.180.22.6039-6042.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We isolated and characterized traD mutants with an altered specificity of interaction with relaxosomes of various conjugative (F and R388) and mobilizable (RSF1010 and ColE1) plasmids. The change in specificity was due to a loss of some amino acids in the carboxyl terminus of TraD that resulted in a broadening of the range of mobilizable relaxosomes at the expense of a decrease in the efficiency of F-plasmid transfer.
引用
收藏
页码:6039 / 6042
页数:4
相关论文
共 17 条
[1]   ESSENTIAL MOTIFS OF RELAXASE (TRAI) AND TRAG PROTEINS INVOLVED IN CONJUGATIVE TRANSFER OF PLASMID-RP4 [J].
BALZER, D ;
PANSEGRAU, W ;
LANKA, E .
JOURNAL OF BACTERIOLOGY, 1994, 176 (14) :4285-4295
[2]   CONSTRUCTION AND PROPERTIES OF A FAMILY OF PACYC184-DERIVED CLONING VECTORS COMPATIBLE WITH PBR322 AND ITS DERIVATIVES [J].
BARTOLOME, B ;
JUBETE, Y ;
MARTINEZ, E ;
DELACRUZ, F .
GENE, 1991, 102 (01) :75-78
[3]   CONJUGATIVE TRANSFER BY THE VIRULENCE SYSTEM OF AGROBACTERIUM-TUMEFACIENS [J].
BEIJERSBERGEN, A ;
DENDULKRAS, A ;
SCHILPEROORT, RA ;
HOOYKAAS, PJJ .
SCIENCE, 1992, 256 (5061) :1324-1327
[4]   Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation [J].
Cabezon, E ;
Sastre, JI ;
delaCruz, F .
MOLECULAR & GENERAL GENETICS, 1997, 254 (04) :400-406
[5]   REQUIREMENTS FOR MOBILIZATION OF PLASMIDS RSF1010 AND COLE1 BY THE INCW PLASMID-R388 - TRWB AND RP4-TRAG ARE INTERCHANGEABLE [J].
CABEZON, E ;
LANKA, E ;
DELACRUZ, F .
JOURNAL OF BACTERIOLOGY, 1994, 176 (14) :4455-4458
[6]   BIOCHEMICAL-CHARACTERIZATION OF ESCHERICHIA-COLI DNA HELICASE-I [J].
DASH, PK ;
TRAXLER, BA ;
PANICKER, MM ;
HACKNEY, DD ;
MINKLEY, EG .
MOLECULAR MICROBIOLOGY, 1992, 6 (09) :1163-1172
[7]   GENETIC AND MOLECULAR CHARACTERIZATION OF TN21, A MULTIPLE RESISTANCE TRANSPOSON FROM R100.1 [J].
DELACRUZ, F ;
GRINSTED, J .
JOURNAL OF BACTERIOLOGY, 1982, 151 (01) :222-228
[8]   The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro [J].
DisqueKochem, C ;
Dreiseikelmann, B .
JOURNAL OF BACTERIOLOGY, 1997, 179 (19) :6133-6137
[9]  
FIRTH N, 1996, ESCHERICHIA COLI SAL, V2, P2377
[10]   DIFFERENTIAL PLASMID RESCUE FROM TRANSGENIC MOUSE DNAS INTO ESCHERICHIA-COLI METHYLATION-RESTRICTION MUTANTS [J].
GRANT, SGN ;
JESSEE, J ;
BLOOM, FR ;
HANAHAN, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4645-4649