Secondary bile acid induced DNA damage in HT29 cells: Are free radicals involved?

被引:54
作者
Booth, LA [1 ]
Gilmore, IT [1 ]
Bilton, RF [1 ]
机构
[1] ROYAL LIVERPOOL UNIV HOSP,DEPT GASTROENTEROL,LIVERPOOL L3 3BX,MERSEYSIDE,ENGLAND
关键词
SCGE assay; bile acid; DNA damage; antioxidants;
D O I
10.3109/10715769709097792
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Increased bile acid secretion, as a consequence of a high fat diet, results in the increased production of bile acids that may escape the enterohepatic circulation, and be subsequently metabolised by the colonic micro-flora to form the co-mutagenic and co-carcinogenic secondary bile acids. The potential of the secondary bile acids lithocholate (LOC) and deoxycholate (DOG), to induce DNA damage, in the colonocyte cell line HT29, at physiological concentrations both individually and in a 2:1 ratio was assessed. Results indicated significant levels of DNA damage induced by both bile acids, with LOC having the greater DNA damaging capacity. The potential role of vitamin A, and the antioxidant vitamin E, in reducing this damage was determined, over a range of vitamin concentrations. Both vitamins reduced the bile acid induced DNA damage. Vitamin A displayed a dose response relationship, whereas vitamin E reduced DNA damage close to negative control values at all concentrations above 50 mu M. These results indicate a protective role for Vitamins A and E, against the DNA damaging capacity of LOC and DOC.
引用
收藏
页码:135 / 144
页数:10
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