Acridones and quinacridones: Novel fluorophores for fluorescence lifetime studies

被引：51

作者：

Smith, JA ^{[1
]}

West, RM ^{[1
]}

Allen, M ^{[1
]}

机构：

[1] Amersham Plc, Amersham Biosci, Maynard Ctr, Forest Farm, Cardiff CF14 7YT, S Glam, Wales

来源：

JOURNAL OF FLUORESCENCE | 2004年 / 14卷 / 02期

关键词：

fluorescence lifetime; spectroscopic characteristics; photostability; labelled biomolecules;

D O I：

10.1023/B:JOFL.0000016287.56322.eb

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two new families of fluorescent probe, acridones and quinacridones, whose fluorescence lifetime can be altered to produce a range of lifetimes from 3 ns to 25 ns are described. Both families of fluorophore have fluorescence lifetimes which are unaffected by pH in the range of 5 to 9 and show a marked resistance to photobleaching. The probes have been modified to allow them to be attached to biomolecules and the labelling of a neuropeptide (substance P) is described. The labelled peptides have the same fluorescence lifetime as the free fluorophore. Quinacridone, with an emission around 550 nm offers a long fluorescence lifetime, photostable alternative to fluorescein.

引用

页码：151 / 171

页数：21

共 53 条

[1]

Acheson R.M., 1973, ACRIDINES

[2] THE BROMINATION OF ACRIDONE [J].

ACHESON, RM ;

ROBINSON, MJT .

JOURNAL OF THE CHEMICAL SOCIETY, 1953, (JAN) :232-238

[3] Linker-mediated modulation of the chemiluminescent signal from N10-(3-sulfopropyl)-N-sulfonylacridinium-9-carboxamide tracers [J].

Adamczyk, M ;

Chen, YY ;

Fishpaugh, JR ;

Mattingly, PG ;

Pan, Y ;

Shreder, K ;

Yu, ZG .

BIOCONJUGATE CHEMISTRY, 2000, 11 (05) :714-724

[4]

Albert A, 1942, ORG SYNTH, V22, P5

[5] IN-VITRO ACTIVITIES OF FUROQUINOLINE AND ACRIDONE ALKALOIDS AGAINST PLASMODIUM-FALCIPARUM [J].

BASCO, LK ;

MITAKU, S ;

SKALTSOUNIS, AL ;

RAVELOMANANTSOA, N ;

TILLEQUIN, F ;

KOCH, M ;

LEBRAS, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (05) :1169-1171

[6]

BERNDT K, 1987, Z PHYS CHEM-LEIPZIG, V268, P250

[7] 3,5-diaryl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) dyes:: Synthesis, spectroscopic, electrochemical, and structural properties [J].

Burghart, A ;

Kim, HJ ;

Welch, MB ;

Thoresen, LH ;

Reibenspies, J ;

Burgess, K ;

Bergstrom, F ;

Johansson, LBÅ .

JOURNAL OF ORGANIC CHEMISTRY, 1999, 64 (21) :7813-7819

[8] Lifetime- and color-tailored fluorophores in the micro- to millisecond time regime [J].

Chen, JY ;

Selvin, PR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (04) :657-660

[9] Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye-DNA complexes [J].

Cosa, G ;

Focsaneanu, KS ;

McLean, JRN ;

Scaiano, JC .

CHEMICAL COMMUNICATIONS, 2000, (08) :689-690

[10] A novel acridone derivative for the fluorescence tagging and mass spectrometric sequencing of peptides [J].

Faller, T ;

Hutton, K ;

Okafo, G ;

Gribble, A ;

Camilleri, P ;

Games, DE .

CHEMICAL COMMUNICATIONS, 1997, (16) :1529-1530

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