Magnetic Nanoparticles Coated with Thiomannosides or Iminosugars to Switch and Recycle Galactosidase Activity

被引：12

作者：

Alvarez-Dorta, Dimitri ^{[1
]}

Brissonnet, Yoan ^{[1
]}

Saumonneau, Amelie ^{[2
]}

Deniaud, David ^{[1
]}

Bernard, Julien ^{[3
,4
,5
]}

Yan, Xibo ^{[3
,4
,5
]}

Tellier, Charles ^{[2
]}

Daligault, Franck ^{[2
]}

Gouin, Sebastien G. ^{[1
]}

机构：

[1] Univ Nantes, CEISAM,UFR Sci & Tech, CNRS,UMR 6230, Chim & Interdisciplinarite Synth Anal Modelisat, 2 Rue Houssiniere,BP 92208, F-44322 Nantes 3, France

[2] Univ Nantes, CNRS, UFIP, UFR Sci & Tech,UMR 6286, 2 Rue Houssiniere,BP 92208, F-44322 Nantes 3, France

[3] Univ Lyon, F-69003 Lyon, France

[4] INSA Lyon, IMP, F-69621 Villeurbanne, France

[5] CNRS, UMR 5223, Ingn Mat Polymeres, F-69621 Villeurbanne, France

来源：

CHEMISTRYSELECT | 2017年 / 2卷 / 29期

关键词：

Enzymatic effectors; Glycosidases; Iminosugars; Magnetic nanoparticles; Multivalency; ALPHA-GALACTOSIDASES; GLYCO-NANOPARTICLES; GOLD NANOPARTICLES; IMMOBILIZATION; GLYCOSIDASES; HYDROLASE; TOOL;

D O I：

10.1002/slct.201702063

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Glycosidase effectors have rarely been reported despite their great potential interest in pharmaceutical sciences and industry. Magnetic nanoparticles were coated with thiomannosides (SMan@Fe3O4) or the broad spectrum glycosidase inhibitor deoxynojirimycin (DNJ@Fe3O4). The coated ligands were shown to exert a fully reverse effect on a model galactosidase (AgaB), with SMan@Fe3O4 or DNJ@Fe3O4 ligands acting as an enzyme inhibitor (K-i = 3.7 mu M) or a strong activator (250% higher AgaB velocity at 50 mu M), respectively. This is striking considering that monovalent soluble SMan and DNJ analogues do not interact with AgaB at millimolar concentrations. The AgaB-DNJ@Fe3O4 enzyme-effector complex could be magnetically recycled and still showed a higher activity compared to free AgaB after four catalytic cycles. The "boost and recycle" procedure may provide interesting perspectives in glycosidase biocatalysis.

引用

页码：9552 / 9556

页数：5

共 22 条

[1] Iminosugar-based glycopolypeptides: glycosidase inhibition with bioinspired glycoprotein analogue micellar self-assemblies [J].