Visualization of growth signal transduction cascades in living cells with genetically encoded probes based on Forster resonance energy transfer

被引：34

作者：

Aoki, Kazuhiro ^{[1
]}

Kiyokawa, Etsuko ^{[1
]}

Nakamura, Takeshi ^{[1
]}

Matsuda, Michiyuki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto 6068501, Japan

来源：

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES | 2008年 / 363卷 / 1500期

关键词：

Forster resonance energy transfer; fluorescence resonance energy transfer; fluorescence protein; signal transduction;

D O I：

10.1098/rstb.2008.2267

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fluorescence probes based on the principle of Forster resonance energy transfer ( FRET) have shed new light on our understanding of signal transduction cascades. Among them, unimolecular FRET probes containing fluorescence proteins are rapidly increasing in number because these genetically encoded probes can be easily loaded into living cells and allow simple acquisition of FRET images. We have developed probes for small GTPases, tyrosine kinases, serine-threonine kinases and phosphoinositides. Images obtained with these probes have revealed that membrane protrusions such as nascent lamellipodia or neurites provide an active signalling platform in the growth factor-stimulated cells.

引用

页码：2143 / 2151

页数：9

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