Determination of clenbuterol, ractopamine and zilpaterol in liver and urine by liquid chromatography tandem mass spectrometry

被引：184

作者：

Blanca, J ^{[1
]}

Muñoz, P ^{[1
]}

Morgado, M ^{[1
]}

Méndez, N ^{[1
]}

Aranda, A ^{[1
]}

Reuvers, T ^{[1
]}

Hooghuis, H ^{[1
]}

机构：

[1] Spanish Author Food Safety, Natl Food Ctr, Majadahonda 28220, Madrid, Spain

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 529卷 / 1-2期

关键词：

beta-agonists; analysis; residue; multiresidue; LC-MS-MS;

D O I：

10.1016/j.aca.2004.09.061

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Beta-agonists have been misused as growth promoting agents in meat producing animals over 20 years. Clenbuterol (CLB) was the main beta-agonist substance used illegally for this purpose. But other beta-agonist substances have been developed to be used for zootechnical purposes, such as ractopamine (RCT), licensed for this application in the United States and currently zilpaterol (ZIL), licensed in South Africa and Mexico. However, these compounds are banned in the European Union (EU) Council Directive 96/22/EC. An analytical method able to identify these three beta-agonists by liquid chromatography tandem mass spectrometry (LC-MS-MS), after clean-up with mixed-mode SPE Bond Elut Certify cartridges (6 mL, 300 mg) was developed and validated according to the Commission Decision 2002/657/EC. Decision limit (CCalpha) ranged from 40 to 110 pg mL(-1) and from 80 to 150 pg g(-1) (ppt) for urine and liver, respectively. Detection capability (CCbeta) ranged from 180 to 270 pg mL(-1) and 270 to 520 pg g(-1) (ppt) for urine and liver respectively. This method appeared suitable for the control of these beta-agonists residues in liver and urine. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：199 / 205

页数：7

共 24 条

[21] Smith DJ, 1998, J ANIM SCI, V76, P173
[22] Zilpaterol - a new focus of concern in residue analysis
Stachel, CS
Radeck, W
Gowik, P
[J]. ANALYTICA CHIMICA ACTA, 2003, 493 (01) : 63 - 67
[23] Detection of multi-β-agonist residues in liver matrix by use of a surface plasma resonance biosensor
Traynor, IM
Crooks, SRH
Bowers, J
Elliott, CT
[J]. ANALYTICA CHIMICA ACTA, 2003, 483 (1-2) : 187 - 191
[24] VANGINKEL LA, 1991, P EC WORKSHOP RIVM B, P72

← 1 2 3 →