Detection of multi-β-agonist residues in liver matrix by use of a surface plasma resonance biosensor

被引:65
作者
Traynor, IM [1 ]
Crooks, SRH [1 ]
Bowers, J [1 ]
Elliott, CT [1 ]
机构
[1] Dept Agr & Rural Dev, Vet Sci Div, Biochem Sect, Belfast BT4 3SD, Antrim, North Ireland
关键词
multi-beta-agonist; biosensor; liver matrix;
D O I
10.1016/S0003-2670(03)00256-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The abuse of growth promoting beta-agonist drugs in animal production remains a problem in many parts of the world. Evidence exists that suggests the range of compounds being abused has become more varied over the past number of years. Detection of a wide family of drugs at low concentrations in complex biological matrices poses significant analytical difficulties. The present study outlines a novel approach to overcome some of these problems by using a high-affinity, broad-spectrum p-agonist monoclonal antibody in an immuno-biosensor assay based on surface plasmon resonance. The assay was capable of analysing 16 liver samples for multi-beta-agonists residues within 1.5 days. Liver sample preparation involved proteolytic digestion, enzymatic deconjugation and solid-phase extraction using Oasis(TM) cartridges. The resultant extracts were mixed with the antibody prior to being injected for 2 min over a clenbuterol coated sensor chip. The sensor surface was regenerated using 0.1 M NaOH. In total, inclusive of washing procedures, etc., it took 10 min for the biosensor to analyse a single sample. The detection limit of the assay was determined according to the guidelines laid down for screening assay validation in the recently revised version of EC Directive 96/23. The assay was able to detect mabuterol (MBL) down to 0.02 ng g(-1), clenbuterol at 0.11 ng g(-1) and salbutamol at 0.19 ng g(-1). A wide range of other beta-agonists were also detected at concentrations below 1.5 ng g(-1). (C) 2003 Published by Elsevier Science B.V.
引用
收藏
页码:187 / 191
页数:5
相关论文
共 20 条
  • [1] Detection of streptomycin residues in whole milk using an optical immunobiosensor
    Baxter, GA
    Ferguson, JP
    O'Connor, MC
    Elliott, CT
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (07) : 3204 - 3207
  • [2] Biosensor assay of sulfadiazine and sulfamethazine residues in pork
    Bjurling, P
    Baxter, GA
    Caselunghe, M
    Jonson, C
    O'Connor, M
    Persson, B
    Elliott, CT
    [J]. ANALYST, 2000, 125 (10) : 1771 - 1774
  • [3] APPLICATION OF MATRIX SOLID-PHASE DISPERSION FOR THE DETERMINATION OF CLENBUTEROL IN LIVER SAMPLES
    BOYD, D
    SHEARAN, P
    HOPKINS, JP
    OKEEFFE, M
    SMYTH, MR
    [J]. ANALYTICA CHIMICA ACTA, 1993, 275 (1-2) : 221 - 226
  • [4] DETERMINATION OF CLENBUTEROL IN BOVINE LIVER BY ENZYME-IMMUNOASSAY
    BUCKNALL, SD
    MACKENZIE, AL
    SAUER, MJ
    EVEREST, DJ
    NEWMAN, R
    JACKMAN, R
    [J]. ANALYTICA CHIMICA ACTA, 1993, 275 (1-2) : 227 - 230
  • [5] DETERMINATION OF CLENBUTEROL IN BOVINE-TISSUES AND URINE BY ENZYME-IMMUNOASSAY
    DEGAND, G
    BERNESDUYCKAERTS, A
    MAGHUINROGISTER, G
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (01) : 70 - 75
  • [6] FLEEKER JR, 1985, J ASSOC OFF ANA CHEM, V68, P172
  • [7] Development of a biosensor-based immunoassay for screening of chloramphenicol residues in milk
    Gaudin, V
    Maris, P
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2001, 13 (02) : 77 - 86
  • [8] DETERMINATION OF CLENBUTEROL IN BOVINE PLASMA AND TISSUES BY GAS-CHROMATOGRAPHY NEGATIVE-ION CHEMICAL IONIZATION MASS-SPECTROMETRY
    GIRAULT, J
    FOURTILLAN, JB
    [J]. JOURNAL OF CHROMATOGRAPHY, 1990, 518 (01): : 41 - 52
  • [9] Hanrahan J. P., 1986, Recent advances in animal nutrition - 1986, P125
  • [10] Haughey SA, 2001, J AOAC INT, V84, P1025