A 43 kDa DNA binding protein from the pea chloroplast interacts with and stimulates the cognate DNA polymerase

被引:10
作者
Chen, WL
Gaikwad, A
Mukherjee, SK
Choudhary, NR
Kumar, D
Tewari, KK
机构
[1] INT CTR GENET ENGN & BIOTECHNOL,NEW DELHI 110067,INDIA
[2] JAWAHARLAL NEHRU UNIV,GENET ENGN UNIT,CBT,NEW DELHI 110067,INDIA
[3] UNIV CALIF IRVINE,DEPT MOL BIOL & BIOCHEM,IRVINE,CA 92717
关键词
D O I
10.1093/nar/24.20.3953
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A DNA binding protein with DNA polymerase 'accessory activity' has been identified and purified to apparent homogeneity from pea chloroplasts, This protein consists of a single subunit of 43 kba and binds to DNA regardless of its base sequence and topology. It increases cognate DNA polymerase-primase activity in a dose dependent manner. Using solid phase protein-protein interaction trapping and co-immuno precipitation techniques, the purified protein was found to associate with the chloroplast DNA polymerase. The chloroplast DNA polymerase also binds directly to the radioiodinated 43 kDa protein. The specific interaction between 43 kDa protein and chloroplast DNA polymerase results in the synthesis of longer DNA chains. The 43 kDa protein, present abundantly in the pea chloroplast, appears to increase processivity of the chloroplast DNA polymerase and may play an important role in the replication of pea chloroplast DNA.
引用
收藏
页码:3953 / 3961
页数:9
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