Development of a magnetic microplate chemifluorimmunoassay for rapid detection of bacteria and toxin in blood

被引：22

作者：

Yu, H

Ahmed, H

Vasta, GR

机构：

[1] Veridian Corp, Dayton, OH 45431 USA

[2] Univ Maryland, Columbus Ctr, Baltimore, MD 21202 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 261卷 / 01期

关键词：

D O I：

10.1006/abio.1998.2614

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A magnetic microplate chemifluorimmunoassay (MMCLA) is described using an immunomagnetic separation and a fluorescent microplate technique for rapid detection of low-level Escherichia coli O157:H7, Bacillus subtilis var. niger spores, and Staphylococcal enterotoxin type B from whole blood, In general, the MMCIA has at least several-fold more sensitivity than the conventional enzyme-linked immunosorbent assay. In addition, the assay sensitivities using direct fluorochrome label as the reporter, or alkaline phosphatase (AP) with various assay substrates, such as pNPP and AttoPhos, were assessed. (C) 1998 Academic Press.

引用

页码：1 / 7

页数：7

共 14 条

[1]

BERGDOLL MS, 1970, MICROBIAL TOXINS, P265

[2]

CANO RJ, 1992, BIOTECHNIQUES, V12, P264

[3] Detection of attomolar concentrations of alkaline phosphatase by capillary electrophoresis using laser-induced fluorescence detection [J].