Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources

被引：49

作者：

Di Pinto, A ^{[1
]}

Forte, VT ^{[1
]}

Conversano, MC ^{[1
]}

Tantillo, GM ^{[1
]}

机构：

[1] Univ Studi Bari, Dipartimento Sanita & Benessere Anim, I-70010 Valenzano Bari, Italy

来源：

FOOD CONTROL | 2005年 / 16卷 / 05期

关键词：

D-PCR; species identification; horse; pork; sausages;

D O I：

10.1016/j.foodcont.2004.04.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Species identification in meat products represents an important subject in the field of modern food control according to the European Union, which has implemented a set of very strict procedures to label food. Thus, specific, sensitive and easy analytical methods for the species detection of food are necessary in order to verify the compliance with labelling requirements. A PCR-based assay for the detection of pork meat in horse fresh sausages was optimised and it was used to evaluate the presence of fraudulently added pork meat. The developed assay showed the presence of pork meat in 6/30 and the total absence of horse meat in 1/30 of the analyzed horse sausage samples. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：391 / 394

页数：4

共 13 条

[11] Establishment and application of a fluorescent polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying porcine, caprine, and bovine meats
Sun, YL
Lin, CS
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (07) : 1771 - 1776
[12] Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA
Verkaar, ELC
Nijman, IJ
Boutaga, K
Lenstra, JA
[J]. MEAT SCIENCE, 2002, 60 (04) : 365 - 369
[13] Quantitative competitive (QC) PCR for quantification of porcine DNA
Wolf, C
Lüthy, J
[J]. MEAT SCIENCE, 2001, 57 (02) : 161 - 168

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